alpha1 Integrin cytoplasmic domain is involved in focal adhesion formation via association with intracellular proteins

The Biochemical Journal
K LösterK Danker

Abstract

Integrins are heterodimeric adhesion receptors consisting of alpha- and beta-subunits capable of binding extracellular matrix molecules as well as other adhesion receptors on neighbouring cells. These interactions induce various signal transduction pathways in many cell types, leading to cytoskeletal reorganization, phosphorylation and induction of gene expression. Integrin ligation leads to cytoplasmic protein-protein interactions requiring both integrin cytoplasmic domains, and these domains are initiation points for focal adhesion formation and subsequent signal transduction cascades. In previous studies we have shown that the very short cytoplasmic alpha1 tail is required for post-ligand events, such as cell spreading as well as actin stress-fibre formation. In the present paper we report that cells lacking the cytoplasmic domain of the alpha1 integrin subunit are unable to form proper focal adhesions and that phosphorylation on tyrosine residues of focal adhesion components is reduced on alpha1beta1-specific substrates. The alpha1 cytoplasmic sequence is a specific recognition site for focal adhesion components like paxillin, talin, alpha-actinin and pp125FAK. It seems to account for alpha1-specific signalling, since when ...Continue Reading

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Citations

Feb 12, 2005·Neuroreport·Jose Arturo Enríquez-AragónBulmaro Cisneros
Jun 17, 2010·The Tohoku Journal of Experimental Medicine·Naoki YotsumotoMinesuke Yokoyama
Aug 29, 2006·Journal of Molecular Biology·Joel CernaBulmaro Cisneros
Jun 24, 2015·Molecular and Cellular Biology·Philip J UrenLuiz O F Penalva
Nov 29, 2001·The Journal of Biological Chemistry·Dörte VossmeyerKerstin Danker
Jan 17, 2015·The Journal of Biological Chemistry·Ashley S WilliamsDavid H Wasserman
Sep 29, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Shomron Ben-Horin, Ilan Bank

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