Alpha1B-adrenoceptor signaling and cell motility: GTPase function of Gh/transglutaminase 2 inhibits cell migration through interaction with cytoplasmic tail of integrin alpha subunits

The Journal of Biological Chemistry
Sung Koo KangMie-Jae Im

Abstract

A multifunctional enzyme, G(h), is a GTP-binding protein that couples to the alpha(1B)-adrenoreceptor and stimulates phospholipase C-delta1 but also displays transglutaminase 2 (TG2) activity. G(h)/TG2 has been implicated to play a role in cell motility. In this study we have examined which function of G(h)/TG2 is involved in this cellular response and the molecular basis. Treatment of human aortic smooth muscle cell with epinephrine inhibits migration to fibronectin and vitronectin, and the inhibition is blocked by the alpha(1)-adrenoreceptor antagonist prazosin or chloroethylclonidine. Up-regulation or overexpression of G(h)/TG2 in human aortic smooth muscle cells, DDT1-MF2, or human embryonic kidney cells, HEK 293 cells, results in inhibition of the migratory activity, and stimulation of the alpha(1B)-adrenoreceptor with the alpha(1) agonist further augments the inhibition of migration of human aortic smooth muscle cells and DDT1-MF2. G(h)/TG2 is coimmunoprecipitated by an integrin alpha(5) antibody and binds to the cytoplasmic tail peptide of integrins alpha(5), alpha(v), and alpha(IIb) subunits in the presence of guanosine 5'-3-O-(thio)triphosphate (GTPgammaS). Mutation of Lys-Arg residues in the GFFKR motif, present in th...Continue Reading

References

Dec 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C S GreenbergR H Rice
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S CotecchiaB K Kobilka
Nov 10, 1995·The Journal of Biological Chemistry·K C HwangM J Im
Jul 1, 1993·The Journal of Cell Biology·J S BauerR L Juliano
Apr 17, 1995·Biochemical and Biophysical Research Communications·N IwaiM Kinoshita
Jan 27, 1998·The Journal of Biological Chemistry·J ZhangG V Johnson
Apr 23, 1999·The Journal of Biological Chemistry·R VezzaG A FitzGerald
Sep 11, 1999·The International Journal of Biochemistry & Cell Biology·J S Chen, K Mehta
Apr 6, 2000·The Journal of Biological Chemistry·J M TaylorJ T Parsons
Oct 9, 2002·The Biochemical Journal·Martin GriffinCarlo M Bergamini
Feb 4, 2003·Nature Reviews. Molecular Cell Biology·Laszlo Lorand, Robert M Graham
Mar 22, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Kyung-Hye LeeKi-Chul Hwang
Apr 19, 2003·Circulation Research·Zhibing ZhangGarret A FitzGerald
May 24, 2003·Biochemical and Biophysical Research Communications·Zee-Won LeeKwon-Soo Ha
Sep 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karkada MohanThomas B Issekutz

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Citations

Aug 6, 2011·Amino Acids·Zhuo Wang, Martin Griffin
Oct 12, 2011·Amino Acids·Manaswini SivaramakrishnanTristan I Croll
Jan 19, 2008·Circulation Research·Kristen A JohnsonRobert A Terkeltaub
Jun 12, 2008·Journal of Cell Science·Kristen A JohnsonRobert A Terkeltaub
May 30, 2009·PloS One·Stephanie W WattsJanice M Thompson
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·David C SaneCharles S Greenberg
Jun 30, 2007·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Mikhail G DozmorovHsueh-Kung Lin
Dec 3, 2016·Biochemical and Biophysical Research Communications·Alina MonteagudoGail V W Johnson
Mar 17, 2007·Circulation Research·William T Gerthoffer
Feb 5, 2005·The Journal of Biological Chemistry·Kristen A Johnson, Robert A Terkeltaub
Jan 1, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·K C Penumatsa, B L Fanburg
Apr 3, 2014·Physiological Reviews·Richard L EckertKapil Mehta
Feb 19, 2011·Molecular Cancer Therapeutics·May KhannaSamy Meroueh
Jun 12, 2021·Frontiers in Cell and Developmental Biology·Dianne M Perez

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