alpha2-Adrenoceptor stimulation promotes actin polymerization and focal adhesion in 3T3F442A and BFC-1beta preadipocytes

Endocrinology
S BétuingJ S Saulnier-Blache

Abstract

We previously demonstrated that in white fat cell precursors alpha2-adrenoceptor stimulation lead to the phosphorylation of p44 and p42 mitogen-activated protein kinases and an increase in cell number. Regulation of cell adhesion and cell cytoskeleton plays a crucial role in the control of cell growth by various growth factors. Here, we report that in mouse 3T3F442A preadipocytes expressing 2500 fmol/mg protein of the human alpha2C10-adrenoceptor (alpha2AF2 cells), alpha2-adrenergic stimulation rapidly restored the spreading of cells previously retracted by serum withdrawal. This effect was pertussis toxin sensitive and was blocked by pretreatment of the cells with dihydrocytochalasin B (a blocker of actin polymerization), genistein (a tyrosine kinase inhibitor), or agents that increase cell cAMP content. Spreading was accompanied by cell membrane ruffling, formation of lamelipodia and filipodia, appearance of focal adhesion plaques, and induction of actin stress fibers. alpha2-Adrenergic stimulation also lead to a rapid Gi- and actin-dependent tyrosine phosphorylation of the pp125 focal adhesion kinase (FAK) as well as of the p42 and p44 mitogen-activated protein kinases. alpha2-Adrenergic-dependent spreading and FAK and mitog...Continue Reading

Citations

Jul 8, 1999·The American Journal of Pathology·J ZhengP Roy-Burman
Nov 24, 1999·Biochemical and Biophysical Research Communications·C PagèsJ S Saulnier-Blache
Jul 25, 2014·American Journal of Physiology. Renal Physiology·Thomas KunitMartin Hennenberg
Dec 2, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J L Rodríguez-Fernández
May 20, 2000·Annals of the New York Academy of Sciences·G PagèsJ S Saulnier-Blache
Nov 30, 2006·American Journal of Physiology. Endocrinology and Metabolism·Jessica B FlowersAlan D Attie
Jun 23, 1998·The Journal of Biological Chemistry·S BétuingJ S Saulnier-Blache
Jun 27, 1997·Biochemical and Biophysical Research Communications·S BétuingJ S Saulnier-Blache
Nov 28, 2021·Cell and Tissue Research·Takaomi ShimokawaKozo Yoneda

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