Abstract
Glycosyltransferase gene transfection into cell lines has been an approach used successfully to elucidate the functional role of cell surface glycoconjugates. We have transfected the rat CMP-NeuAc:Galbeta1,4GlcNAc alpha2,6-sialyltransferase (EC 2.4.99.1) gene into a human, tumorigenic, glioma cell line, U373 MG. This transfection led to a marked inhibition of invasivity, alterations in adhesivity to fibronectin and collagen matrices, and inappropriately sialylated alpha3beta1 integrin. Adhesion-mediated protein tyrosine phosphorylation was reduced in the transfectants despite increased expression of focal adhesion kinase, p125fak. Furthermore, the transfectants showed a distinct cell morphology, an increased number of focal adhesion sites, and different sensitivity to cytochalasin D treatment than control U373 MG cells. These results suggest that inappropriate sialylation of cell surface glycoconjugates, such as integrins, can change focal adhesion as well as adhesion-mediated signal transduction and block glioma cell invasivity in vitro.
Citations
Mar 23, 2005·Brain Pathology·Lars TatenhorstWerner Paulus
Aug 26, 2016·Carcinogenesis·Yu Ri JungMinyoung Lee
Nov 24, 1999·Journal of Neuroscience Research·N Georgopoulou, K C Breen
Dec 6, 2007·Journal of Neurochemistry·Roger A KroesJoseph R Moskal
Oct 17, 2009·Expert Review of Neurotherapeutics·Joseph R MoskalGlyn Dawson
Jun 11, 2004·Journal of Neurochemistry·G DawsonS A Dawson
Apr 22, 2008·Neurosurgery·Johann MicallefAbhijit Guha
Jun 13, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takeshi Sato, Kiyoshi Furukawa
Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ying-Chih LiuChi-Huey Wong
Aug 20, 2014·Bioscience Reports·Séverine Van SlambrouckWim F A Steelant
Dec 7, 2013·Gut and Liver·Jung-Jin Park, Minyoung Lee
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Roger A KroesJoseph R Moskal
Aug 13, 2013·PloS One·Brock SchumanThomas M Fyles
Sep 5, 2012·The FEBS Journal·Maho AmanoHans-Joachim Gabius
Aug 12, 2016·Glycobiology·Roger A Kroes, Joseph R Moskal
Sep 30, 2005·Glycobiology·Mariella ChiricoloFabio Dall'Olio
Jul 19, 2019·Glycobiology·Giulia VenturiFabio Dall'Olio
Aug 19, 2008·Molecular Cancer Research : MCR·Minyoung LeeYun-Sil Lee
Dec 16, 2020·Glycobiology·Kaitlyn A DorsettSusan L Bellis
Feb 24, 2016·Life Sciences·Ye YuanMonica M Burdick
May 26, 2004·Biochimica Et Biophysica Acta·Susan L Bellis
Mar 28, 2006·Biochemical and Biophysical Research Communications·Xiaoning ChenJianxin Gu
Oct 6, 2017·ACS Chemical Neuroscience·Lucas VeillonYehia Mechref
Sep 6, 2007·Journal of Agricultural and Food Chemistry·Simone Rochfort, Joe Panozzo