alpha(2B)-Adrenergic receptors activate MAPK and modulate proliferation of primary cultured proximal tubule cells

American Journal of Physiology. Renal Physiology
Daniel CussacHervé Paris

Abstract

In the rat proximal tubule, the alpha(2B)-adrenergic receptor (alpha(2B)-AR) enhances Na(+) reabsorption by increasing the activity of Na(+)/H(+) exchanger isoform NHE3. The mechanisms involved are unclear, and inhibition of cAMP production remains controversial. In this study, we reinvestigated alpha(2B)-AR signaling pathways using rat proximal tubule cells (PTC) in primary culture and LLC-PK(1) cells permanently transfected with the RNG gene (rat nonglycosylated alpha(2)-AR). Binding experiments indicated that PTC express substantial amounts of alpha(2B)-AR (130 fmol/mg protein), and only RNG transcripts were detected. In both cell types, the alpha(2B)-AR is coupled to G protein, and its stimulation by dexmedetomidine, but not by UK-14304, provoked a significant inhibition of the accumulation of cAMP induced by forskolin or parathyroid hormone. Exposure to alpha(2)-agonists increased arachidonic acid release and caused extracellular signal-regulated kinase (ERK)1/2 phosphorylation, which correlated with enhanced mitogen-activated protein kinse (MAPK) activity and nuclear translocation. MAPK phosphorylation was blunted by pertussis toxin but not by protein kinase C desensitization, and it coincided with transient phosphorylati...Continue Reading

References

Nov 1, 1991·The American Journal of Physiology·G YasudaW B Jeffries
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D W ZengK R Lynch
Nov 1, 1987·The American Journal of Physiology·P R SundaresanS L Kelvie
Dec 1, 1987·The Journal of Clinical Investigation·E P NordP A Insel
Jul 1, 1985·The American Journal of Physiology·S UmemuraW A Pettinger
Feb 1, 1987·The American Journal of Physiology·R E BeachV W Dennis
Oct 1, 1981·The American Journal of Physiology·P VinayG Lemieux
Jun 1, 1993·Hypertension·D E HandyH Gavras
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·W J KochR J Lefkowitz
Sep 1, 1994·The American Journal of Physiology·L E HeasleyT Berl
Jan 1, 1993·Annual Review of Pharmacology and Toxicology·R R RuffoloJ P Hieble
Jan 1, 1996·Fundamental & Clinical Pharmacology·M C Michel, L C Rump
Jan 22, 1998·The American Journal of Physiology·M D OkusaA J Mangrum
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·H JiaoJ G Douglas
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·N O DulinJ G Douglas
Aug 13, 1999·The American Journal of Physiology·J F di MariR L Safirstein

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Citations

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Dec 6, 2014·Acta Anaesthesiologica Taiwanica : Official Journal of the Taiwan Society of Anesthesiologists·Ankur KhajuriaDaqing Ma
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