PMID: 9181835Mar 1, 1997Paper

Alpha/beta and gamma/delta T-cell receptors--analysis of conjunctival tissue of patients with ocular pemphigoid

Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
A A BialasiewiczG Richard

Abstract

In the conjunctiva of asymptomatic persons and patients with ocular cicatricial pemphigoid the existence and distribution of alpha/beta- and gamma/delta-T cell receptor (TCR)-positive cells has not previously been investigated. Biopsy specimens from 20 patients with clinically diagnosed ocular cicatricial pemphigoid (OCP) were compared with 20 specimens from asymptomatic persons. Additionally, 3 specimens from patients with OCP treated with cytotoxic drugs and 8 specimens from patients who had undergone mucosal transplantation were studied. Antigen retrieval was done in deparaffinized specimens for immunohistochemical identification of alpha/beta- and gamma/delta-TCR-positive cells by monoclonal antibodies and an appropriate detection system. All steps were performed in triplicate, and negative control sera were applied. alpha/beta-TCR-positive cells were observed in the conjunctival epithelium and stroma of all the asymptomatic persons and in all patients with OCP. gamma/delta-TCR-positive cells were found in 4 out of 20 patients with OCP in epithelial sites, but not in asymptomatic persons. Patients with OCP who had received cytotoxic treatment exhibited neither alpha/beta- nor gamma/delta-TCR-positive cells. Staining sites o...Continue Reading

Citations

Jun 6, 2006·Allergologia et immunopathologia·A AlonsoR Brunzini

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