Alpha(v) integrin, p38 mitogen-activated protein kinase, and urokinase plasminogen activator are functionally linked in invasive breast cancer cells

The Journal of Biological Chemistry
J ChenS Huang

Abstract

We reported previously that endogenous p38 MAPK activity is elevated in invasive breast cancer cells and that constitutive p38 MAPK activity is important for overproduction of uPA in these cells (Huang, S., New, L., Pan, Z., Han, J., and Nemerow, G. R. (2000) J. Biol. Chem. 275, 12266-12272). However, it is unclear how elevated endogenous p38 MAPK activity is maintained in invasive breast cancer cells. In the present study, we found that blocking alpha(v) integrin functionality with a function-blocking monoclonal antibody or down-regulating alpha(v) integrin expression with alpha(v)-specific antisense oligonucleotides significantly decreased the levels of active p38 MAPK and inhibited cell-associated uPA expression in invasive breast cancer MDA-MB-231 cells. These results suggest a function link between alpha(v) integrin, p38 MAPK activity, and uPA expression in invasive tumor cells. We also found that vitronectin/alpha(v) integrin ligation specifically induced p38 MAPK activation and uPA up-regulation in invasive MDA-MB-231 cells but not in non-invasive MCF7 cells. Finally, using a panel of melanoma cells, we demonstrated that the cytoplasmic tail of alpha(v) integrin subunit is required for alpha(v) integrin ligation-induced ...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R E SeftorM J Hendrix
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R PytelaE Ruoslahti
Jul 15, 1988·International Journal of Cancer. Journal International Du Cancer·S Pereyra-AlfonsoE Bal de Kier Joffé
Apr 22, 1994·Science·P C BrooksD A Cheresh
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·A M MontgomeryD A Cheresh
Mar 1, 1995·Molecular Biology of the Cell·X Zhu, R K Assoian
May 29, 1995·International Journal of Cancer. Journal International Du Cancer·C DugganM J Duffy
Feb 15, 1993·Cancer·S AznavoorianL A Liotta
Jul 29, 1996·International Journal of Cancer. Journal International Du Cancer·R H Xing, S A Rabbani
Jan 1, 1996·Breast Cancer Research and Treatment·D F AlonsoE Bal de Kier Joffé
Nov 15, 1997·Cancer·E C WoodhouseL A Liotta
Mar 12, 1998·International Journal of Cancer. Journal International Du Cancer·A R FarinaA R Mackay
Apr 9, 1998·International Journal of Cancer. Journal International Du Cancer·S SonoharaM M Brentani
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·G J WangJ R Bender
Jun 25, 1998·International Journal of Cancer. Journal International Du Cancer·C HaeckelA Roessner
Dec 2, 1999·Experimental Cell Research·J EnglishM H Cobb
Oct 29, 2000·Cell Adhesion and Communication·S A Wilcox-AdelmanP J McKeown-Longo
Dec 31, 2000·The Journal of Biological Chemistry·P A KlekotkaM M Zutter
Feb 15, 2001·International Journal of Cancer. Journal International Du Cancer·A M KhatibP Brodt
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·B Felding-HabermannB M Mueller

❮ Previous
Next ❯

Citations

Jul 15, 2011·BMC Cancer·Aliakbar TaherianThomas A Haas
Sep 10, 2014·Nucleic Acids Research·Gabriel Moquet-TorcyIsabelle Jariel-Encontre
May 7, 2002·International Journal of Cancer. Journal International Du Cancer·Jun NiuMichael V Agrez
Nov 6, 2008·Pigment Cell & Melanoma Research·Yeriel EstradaLiliana Ossowski
Nov 26, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Rui WangEn-Hua Wang
Jul 18, 2002·Nature Reviews. Drug Discovery·Massimo CristofanilliGabriel N Hortobagyi
Jan 2, 2003·Nature Reviews. Cancer·Patricia S Steeg
Jun 23, 2005·British Journal of Cancer·S E BojesenB G Nordestgaard
Sep 6, 2005·Breast Cancer Research and Treatment·Sophya KonstantinovskyBen Davidson
Dec 18, 2002·Annals of the New York Academy of Sciences·Julie LaferriereJacques Huot
Jun 22, 2014·The Journal of Biological Chemistry·Heesung ChungEok-Soo Oh
Aug 21, 2020·Investigative Ophthalmology & Visual Science·Ping YangGlenn J Jaffe
May 26, 2004·Critical Reviews in Oncology/hematology·G KleinE S J M de Bont
Nov 22, 2007·Neoplasia : an International Journal for Oncology Research·Laetitia DevyPaula Henderikx
Dec 17, 2009·Cancer Letters·Yunfei ZhangQingyu Fan

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.