Alpinetin exerts anti-colitis efficacy by activating AhR, regulating miR-302/DNMT-1/CREB signals, and therefore promoting Treg differentiation

Cell Death & Disease
Qi LvZhi-Feng Wei

Abstract

Alpinetin, a flavonoid compound extracted from the seeds of Alpinia katsumadai Hayata, has been demonstrated to exert massive biological properties. This study aimed to evaluate the effect of alpinetin on dextran sulfate sodium (DSS)-induced colitis, and elucidate the potential mechanisms. Alpinetin significantly alleviated colitis in mice, accompanied with restored Th17/Treg balance in colons. In vitro, alpinetin directly promoted Treg differentiation but exerted little effect on Th17 differentiation, and the action was in an aryl hydrocarbon receptor (AhR)-dependent manner. It acted as a potential AhR activator, evidenced by increased expression of CYP1A1, dissociation of AhR/HSP90 complexes, AhR nuclear translocation, XRE-driven luciferase reporter gene and DNA-binding activity of AhR/ARNT/XRE in T cells. Furthermore, alpinetin significantly promoted expression of miR-302 but not others, and restrained expression of DNMT-1 and methylation level of Foxp3 promoter region in CD4+ T cells and colons of colitis mice. However, the association of CREB and Foxp3 promoter region but not expression, nuclear translocation and DNA-binding activity of CREB was up-regulated by alpinetin in CD4+ T cells. The relationship of alpinetin-adjus...Continue Reading

References

Dec 4, 2003·Environmental Health Perspectives·Shu ZhangStephen H Safe
Jun 27, 2007·The Journal of Experimental Medicine·Hyoung-Pyo Kim, Warren J Leonard
Mar 26, 2008·Nature·Francisco J QuintanaHoward L Weiner
Aug 15, 2008·Cytokine·Irene Pedersen, Michael David
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Girdhari LalJonathan S Bromberg
Jul 31, 2009·Blood·Girdhari Lal, Jonathan S Bromberg
Aug 25, 2009·Trends in Immunology·Charlotte EsserBrigitta Stockinger
Apr 30, 2010·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Guowen ZhangJiao Tian
Mar 5, 2011·Digestive Diseases and Sciences·Keisuke FurumatsuMasaru Yoshida
Oct 25, 2011·Molecular Aspects of Medicine·Dayong WuSimin Nikbin Meydani
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Taisuke NakahamaTadamitsu Kishimoto
Jul 16, 2013·Toxicon : Official Journal of the International Society on Toxinology·Xiaoyu LiYuanyuan Li
Aug 3, 2013·Nature Reviews. Immunology·Dirk Baumjohann, K Mark Ansel
Nov 29, 2013·Current Opinion in Gastroenterology·Wojciech BlonskiGary R Lichtenstein
Dec 4, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chihiro OgawaMasahide Tone
Jun 27, 2014·Immunopharmacology and Immunotoxicology·Shuang GuanJing Lu
Oct 2, 2014·Archives of Pharmacal Research·Ying ZouXue-Bao Zheng
Dec 6, 2014·Gut·R KallaJ Satsangi
Dec 3, 2015·Nature Reviews. Gastroenterology & Hepatology·Heitor S P de Souza, Claudio Fiocchi
Dec 30, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Narendra P SinghMitzi Nagarkatti
Mar 8, 2016·Inflammatory Bowel Diseases·Xuelian HuYong Zhao
Jul 6, 2016·Journal of Hematology & Oncology·Gilles FransoletFrédéric Baron
Dec 5, 2016·Lancet·Ryan UngaroJean-Frédéric Colombel
Apr 30, 2017·Nature Communications·Sonja ReißigNadine Hövelmeyer
Sep 9, 2017·International Immunopharmacology·Guang LiLi-Qiong Lv

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Citations

Jan 30, 2020·Natural Product Reports·Levi W EvansBradley S Ferguson
Jun 21, 2020·Journal of Cellular and Molecular Medicine·Tao ZhangChangwei Qiu
Sep 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jieying QiuZheng Xiang
Jul 4, 2020·Mediators of Inflammation·Xueyan DingGuoqiang Zhu
Sep 10, 2020·International Journal of Molecular Sciences·Anna WajdaAgnieszka Paradowska-Gorycka
Oct 23, 2019·Signal Transduction and Targeted Therapy·Ziying ZhangYongguang Tao
Jan 6, 2021·The Journal of Pharmacology and Experimental Therapeutics·Guolin ZhangDapeng Chen
Oct 11, 2020·Signal Transduction and Targeted Therapy·Ziying ZhangYongguang Tao
Apr 4, 2021·Nutrients·Jian TanLaurence Macia
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elizabeth Goya-JorgeRosa M Giner
Jul 13, 2021·Frontiers in Immunology·Izzah BungsuAnne Catherine Cunningham
Jul 31, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yaoyao ChangHaitao Xiao
Apr 6, 2020·Food Research International·Iramaia Angelica Neri-Numa, Glaucia Maria Pastore
Aug 6, 2019·Journal of Agricultural and Food Chemistry·Xichun XiaJunqing Huang
Jan 1, 2022·BMJ Open Gastroenterology·Julie P SaikiBerkeley Limketkai

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Methods Mentioned

BETA
ELISA
flow cytometry
Co-immunoprecipitation
nuclear translocation
PCR
ChIP
enzyme-linked immune sorbent assay
electrophoresis

Software Mentioned

FlowJo7
SPSS

Related Concepts

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