Alpinumisoflavone rescues glucocorticoid-induced apoptosis of osteocytes via suppressing Nox2-dependent ROS generation

Pharmacological Reports : PR
Jun YinWei Cong

Abstract

Long term use of glucocorticoids is one of the most common causes of secondary osteoporosis. Osteocyte, the most abundant cell type in bone, coordinates the function of osteoblast and osteoclast. This study evaluates the protective effect of alpinumisoflavone (AIF), a naturally occurring flavonoid compound, on dexamethasone (Dex)-induced apoptosis of osteocytes. MLO-Y4 cell was used as a cell model. The effect of AIF on the cell viability was assessed by MTT assay. Apoptosis of MYL-Y4 cells was determined by DNA fragment detection ELISA kit and flow cytometry. Intracellular ROS level was determined by DCFH-DA staining. mRNA and protein expression of target genes were determined by qRT-PCR and western blot, respectively. AIF effectively protected MLO-Y4 cells against Dex-induced apoptosis, which was associated with attenuation of Dex-induced ROS generation in MLO-Y4 cells. Furthermore, our data indicated that the expression of NAD(P)H oxidase 2 (Nox2) was suppressed by AIF, which in turn mediated the attenuating effect on Dex-induced ROS generation and apoptosis in MLO-Y4 cells. Moreover, our results showed that AIF modulated the expression of Nox2 by activating AMPK signaling. AIF activated AMPK-dependent Nox2 signaling pathway...Continue Reading

References

Mar 1, 1990·Annals of Internal Medicine·B P Lukert, L G Raisz
Oct 23, 1997·European Journal of Endocrinology·I R Reid
Jan 26, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·O VerborgtM B Schaffler
Jun 7, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S C Manolagas
Feb 22, 2001·Free Radical Biology & Medicine·M C PolidoriH Sies
Dec 1, 2001·Bone·D PatschanF Buttgereit
Jul 13, 2002·Annals of the New York Academy of Sciences·Ernesto Canalis, Anne M Delany
Mar 26, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yan LiuSylvia Christakos
Jun 13, 2006·Trends in Endocrinology and Metabolism : TEM·Blerina KolaMárta Korbonits
Sep 8, 2006·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·E Seeman
Feb 19, 2008·Bone·Lynda F Bonewald, Mark L Johnson
Apr 26, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Giolanta KogianniBrendon S Noble
Nov 17, 2009·Biochimica Et Biophysica Acta·J J P PerryJ A Tainer
Jun 26, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yukiko KitaseLynda F Bonewald
Jul 14, 2010·Phytotherapy Research : PTR·J C ChukwujekwuJ Van Staden
Jun 28, 2014·European Journal of Pharmacology·Guang-Zhi DongYoung Woo Kim
Sep 6, 2014·Vitamins and Hormones·Jisha JoshuaRenate B Pilz
Jun 12, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Victor KueteThomas Efferth
Dec 13, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yongfeng GuoLi Sun
Jun 27, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wei CongJun Yin

❮ Previous
Next ❯

Citations

Feb 27, 2019·Current Osteoporosis Reports·Robert J PignoloAbhishek Chandra
Sep 26, 2019·Frontiers in Pharmacology·Sylvin Benjamin AtebaDieudonné Njamen
Jan 19, 2021·Pharmacological Reports : PR·UNKNOWN Editorial Office of Pharmacological Reports
Apr 17, 2020·Combinatorial Chemistry & High Throughput Screening·Dong-Jiang LiaoYuan-Sheng Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis