PMID: 7528243Jan 1, 1995Paper

Alteration in cell morphology triggers transforming growth factor-beta 1, collagenase, and tissue inhibitor of metalloproteinases-I expression in normal and hypertrophic scar fibroblasts

The Journal of Investigative Dermatology
M VarediA Ghahary

Abstract

Using immunocytochemistry and Northern blot analysis, we investigated the role of cell morphology and reorganization of the cytoskeleton in the expression of transforming growth factor-beta 1 (TGF-beta 1) in human dermal fibroblasts. Disruption of the cytoskeleton was induced by three different agents--trypsin, ethyl-eneglycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), or cytochalasin--and was confirmed by staining with rhodamine-labeled phalloidin. Immunocytochemical staining with antibodies specific for TGF-beta 1 revealed a cell-shape-related induction of TGF-beta 1. Northern blot analysis of total RNA showed a significant increase in the expression of TGF-beta 1 mRNA as early as 4 h and peaking at 12 h after disruption of the cytoskeleton. Quantitative analysis of TGF-beta 1 mRNA expression at 4 h after treatment with trypsin, EGTA, or cytochalasin C showed increases of 2.6-, 3.3-, and 2.6-fold, respectively. Disruption of the cytoskeleton by trypsin, EGTA, or cytochalasin C increased mRNA for collagenase by 3.8-fold, 2.3-fold, or 2.5-fold, respectively. The expression of mRNA for tissue inhibitor of metalloproteinases I (TIMP-I) also showed a 3.2-fold increase by trypsin, a 3.6-fold increase by EGTA, and...Continue Reading

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Citations

Oct 15, 2009·Journal of Biomedical Materials Research. Part a·Hung-Jen ShaoTai-Horng Young
Feb 12, 1998·The American Journal of Physiology·J M ShannonL D Nielsen
Jun 19, 2007·Plastic and Reconstructive Surgery·Steven L HenryAlberto A Diaz-Arias
Oct 23, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K Tanaka-KamiokaS S Lim
Jun 8, 2000·Annals of the New York Academy of Sciences·E E Tredget
Aug 2, 2001·Biochemical and Biophysical Research Communications·K S Ko, C A McCulloch
Oct 20, 1998·American Journal of Surgery·W K StadelmannG R Tobin
May 14, 1999·Burns : Journal of the International Society for Burn Injuries·J VenugopalM Babu
Jul 11, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Erica H M KerkvlietVincent Everts
Jun 1, 1997·The Surgical Clinics of North America·E E TredgetA Ghahary

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