Alteration in insulin-like growth factor-binding proteins (IGFBPs) and IGFBP-3 protease activity in serum and urine from acute and chronic renal failure

The Journal of Clinical Endocrinology and Metabolism
D Y LeeR G Rosenfeld

Abstract

The insulin-like growth factors, IGF-I and IGF-II, are proteins that promote cellular growth and differentiation of various organs, including the kidney. These peptides interact with high affinity cell surface receptors and bind to a family of IGF-binding proteins (IGFBPs). Altered serum and urinary IGFBP patterns in children with chronic renal failure have been previously described. In this study, we evaluated serum and urinary IGFBP profiles in acute renal failure patients (ARF; n = 10) and chronic renal failure patients (n = 10), using Western ligand blots. Most patients with acute or chronic renal failure showed decreased intact serum IGFBP-3 and increased serum IGFBP-2. Both groups displayed marked urinary IGFBP alterations, including increased urinary IGFBP-1 and totally absent urinary IGFBP-3, as detected by Western ligand blot. To evaluate altered IGFBP profiles, we performed IGFBP-3 protease assays with sera and urine from renal failure patients and normal controls. Although control urine had only minor protease activity (defined by the ability to degrade [125I]IGFBP-3), significant protease activity was found in urine from renal failure patients. The proteolytic pattern and susceptibility to protease inhibitors in mos...Continue Reading

Citations

Sep 16, 2004·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Victoria Schebek-FürstenbergBernd Kübler
Feb 5, 2005·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Burkhard TönshoffSonia Ciarmatori
Jun 12, 2012·Endocrinology and Metabolism Clinics of North America·Daniela Kiepe, Burkhard Tönshoff
Sep 8, 2011·Advances in Chronic Kidney Disease·Halima S Janjua, John D Mahan
Sep 1, 2004·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Jan Frystyk
Jun 7, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Tetyana L VasylyevaRobert J Ferry
Jun 1, 1996·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·B TönshoffO Mehls
Aug 26, 2004·American Journal of Physiology. Renal Physiology·Xuemei WangRodger Loutzenhiser

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