PMID: 36610Apr 1, 1979

Alteration in structure of multifunctional protein from Chinese hamster ovary cells defective in pyrimidine biosynthesis

Proceedings of the National Academy of Sciences of the United States of America
J N Davidson, D Patterson

Abstract

A combined genetic, biochemical, and immunological approach has clarified structural relationships involving the first three enzymes of de novo pyrimidine biosynthesis. A procedure involving antibody and protein A-Sepharose was used to isolate the enzymes carbamoyl-phosphate synthase [ATP:carbamate phosphotransferase (dephosphorylating, amido-transferring), EC 2.7.2.9], aspartate transcarbamoyltransferase (carbamoylphosphate:L-aspartate carbamoyltransferase, EC 2.1.3.2), and dihydro-orotase (L-5,6-dihydroorotate amidohydrolase, EC 3.5.2.3) from Chinese hamster ovary cell CHO-K1, the uridine-requiring auxotroph Urd(-)A, and selected Urd(-)A revertants. The enzymes of Urd(-)A and the Urd(-)A revertants were significantly altered in activity, native structure, and molecular weight from those of CHO-K1. The results presented permit the conclusion that (i) these three enzymes reside in a single multifunctional 220,000-dalton polypeptide; (ii) the aspartate transcarbamoyltransferase activity is located on a portion ( approximately 20,000 daltons) at one end of the polypeptide; (iii) this portion may also be required for monomers to aggregate into the multimeric from present in mammalian cells; (iv) the mutations in Urd(-)A and the Ur...Continue Reading

References

Mar 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J N DavidsonC B Kern
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D PattersonC Jones
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M I MallyD R Evans
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C J Lusty, J Lu
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J N Davidson, L A Niswander
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L LeeD R Evans
Jan 5, 1987·Journal of Molecular Biology·J N Freund, B P Jarry

Citations

Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·R G HAM

Related Concepts

Ovarian Diseases
Chinese Hamster
Enzymes, antithrombotic
Aspartate
Carbamates
Both Ovaries
Peptide Hydrolases
Ovary
Chinese Hamster Ovary Cell
Carbamoyl-Phosphate Synthase I Deficiency Disease

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.