Alteration in the processing of the ACRBP/sp32 protein and sperm head/acrosome malformations in proprotein convertase 4 (PCSK4) null mice.

Molecular Human Reproduction
Steve TardifGail A Cornwall

Abstract

Proprotein convertase 4 (PCSK4) is a member of a family of proprotein convertases that convert inactive precursor proteins into their mature and active forms. PCSK4 is expressed by testicular germ cells and localizes to the sperm acrosome, suggesting roles in fertilization. Mice lacking PCSK4 exhibit a profound fertility defect; yet, to date, few substrates for PCSK4 are known. In this study, two-dimensional differential in-gel electrophoresis analysis was carried out in order to identify proteins that are altered in spermatozoa from PCSK4 null mice. Herein, we report that the sperm fertilization molecule acrosin-binding protein (ACRBP)/sp32, which normally undergoes processing from a 58.5 kDa precursor to a 27.5 kDa mature form, is not proteolytically processed in PCSK4 null mice and thus may be a substrate for PCSK4. However, analysis of the ACRBP sequence did not show a strong consensus site for convertase cleavage, suggesting that ACRBP processing may require the activity of a yet unknown enzyme that itself may be a PCSK4 substrate. Further analysis of spermatozoa from the PCSK4 null mice showed that proacrosin did not undergo autoactivation, supporting a role for the mature form of ACRBP in the regulation of proacrosin con...Continue Reading

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Citations

Jun 19, 2012·Molecular & Cellular Proteomics : MCP·Benoit GuyonnetGail A Cornwall
Jun 16, 2016·Proceedings of the National Academy of Sciences of the United States of America·Yoshinori KanemoriTadashi Baba
Mar 20, 2018·The Journal of Veterinary Medical Science·Osamu Kawase, Mitsuru Jimbo
Aug 1, 2014·Journal of Cellular Physiology·Kessiri KongmanasNongnuj Tanphaichitr
Mar 27, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrew T ReidBrett Nixon
May 28, 2020·International Journal of Molecular Sciences·Maria E TevesPaulene Sapao
May 16, 2020·Biology of Reproduction·Przemysław ZakrzewskiMarta Lenartowska
Mar 22, 2013·Biology of Reproduction·James A Foster
Feb 22, 2013·Biology of Reproduction·Yoshinori KanemoriTadashi Baba
Jan 3, 2021·Histochemistry and Cell Biology·Przemysław ZakrzewskiFolma Buss
Jan 1, 2021·Endocrine Reviews·Manita Shakya, Iris Lindberg
Jun 17, 2021·Biology of Reproduction·Wenfeng XiongZhugang Wang
May 7, 2020·Protein and Peptide Letters·Rossella CannarellaSandro La Vignera

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