Alteration of 11beta-hydroxysteroid dehydrogenase type 1 in skeletal muscle in a rat model of type 2 diabetes.

Molecular and Cellular Biochemistry
Ming ZhangLi Chen

Abstract

Recent investigations have demonstrated that activation of 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) in liver and adipose tissue is closely related to the pathogenesis of obesity and diabetes. However, the relationship between alteration of 11beta-HSD1 and the pathogenesis of type 2 diabetes in skeletal muscle is still unclear. A rat model of Type 2 diabetes was developed by high fat diet feeding combined with multiple low dose streptozotocin injection (30 mg/kg, i.p. twice). Intraperitoneal glucose tolerance test, insulin tolerance test were performed. Fasting blood glucose, fasting insulin, total cholesterol, triglyceride were measured. The protein and mRNA level of 11beta-HSD1 and glucocorticoid receptor in gastrocnemius muscle were determined. The alteration of insulin signaling pathway related protein was investigated. We found that the protein levels of 11beta-HSD1 and glucocorticoid receptor were significantly increased (P < 0.05); the mRNA level of 11beta-HSD1 was also elevated (P < 0.05); the mRNA level of glucocorticoid receptor was decreased (P < 0.05). After insulin stimulation, diabetic rats had no significant changes in the level of the insulin receptor beta-subunit (IR-beta), AKT, as in phosphoryla...Continue Reading

References

Feb 1, 1991·Steroids·K L BurnsteinJ A Cidlowski
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S OkretJ A Gustafsson
Jun 27, 1995·Biochemistry·Y NobukuniS D Detera-Wadleigh
Jan 10, 2003·The Journal of Clinical Endocrinology and Metabolism·Robert C AndrewsBrian R Walker
Mar 22, 2003·The Journal of Pharmacology and Experimental Therapeutics·Dawn E W Livingstone, Brian R Walker
Jan 30, 2004·Recent Progress in Hormone Research·Jonathan R SecklBrian R Walker
Nov 9, 2004·The Journal of Endocrinology·Alistair I FreemanKaren E Chapman
Aug 17, 2005·The Journal of Experimental Medicine·Anne Hermanowski-VosatkaRolf Thieringer
Jan 13, 2006·British Journal of Pharmacology·Julia C Buckingham
Dec 7, 2006·Annals of the New York Academy of Sciences·Brian R Walker, Ruth Andrew
Apr 14, 2007·Current Alzheimer Research·Mary F DallmanMichelle T Foster
May 8, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Nigel VickerBarry V L Potter
Aug 22, 2007·Cell Biochemistry and Biophysics·Håkan K R Karlsson, Juleen R Zierath
Oct 18, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity·Sheridan Henness
Dec 7, 2007·Best Practice & Research. Clinical Endocrinology & Metabolism·Jeremy W Tomlinson, Paul M Stewart

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Citations

Aug 1, 2013·Physiological Reviews·Karen ChapmanJonathan Seckl
Apr 25, 2013·Endocrine Reviews·Laura L GathercolePaul M Stewart
Oct 20, 2009·The Journal of Clinical Endocrinology and Metabolism·Mark S Cooper, Paul M Stewart
Feb 14, 2013·American Journal of Physiology. Endocrinology and Metabolism·J J WinnickD S Edgerton
Apr 7, 2016·The Journal of Endocrinology·Stuart A MorganGareth G Lavery
Jan 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jinyan CaiJiao Guo

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