Alteration of amino acid 101 within capsid protein VP-1 changes the pathogenicity of Theiler's murine encephalomyelitis virus.

The Journal of Experimental Medicine
A ZurbriggenR S Fujinami

Abstract

Chronic Theiler's murine encephalomyelitis virus infection of susceptible mice is an animal model for human demyelinating diseases. Previously we described an altered and diminished pattern of central nervous system disease in immunocompetent SJL/J mice infected with a variant virus. This variant virus H7A6-2 was selected with a neutralizing mAb recognizing the capsid protein VP-1 of Theiler's virus. Here we characterize the variant virus by ELISA and neutralization assays and by sequencing selected regions of the viral RNA genome and relate the alteration to disease. The variant virus contains one single point mutation within a neutralizing epitope of VP-1. This nucleotide change lead to an amino acid replacement at amino acid 101 of VP-1, a threonine (wild type) to an isoleucine (variant). Model building based on sequence alignments and the known structure of the related Mengo virus indicates that the altered amino acid is located in an exposed loop on the surface of the virus at the periphery of a site that has been proposed to be the receptor binding site. The results of ELISA, neutralization assay, and direct RNA sequencing provide for the first time an opportunity to precisely map an important structural determinant of ne...Continue Reading

References

Oct 1, 1976·Journal of the Neurological Sciences·J R LehrichF H Hochberg
Jul 1, 1986·The Journal of General Virology·P D MinorJ P Icenogle
Nov 1, 1988·Journal of Neuroimmunology·R S FujinamiH C Powell
Mar 1, 1986·The Journal of Experimental Medicine·M RodriguezC S David
Jun 1, 1982·Journal of Neuroimmunology·R P RoosB G Arnason
Mar 26, 1982·Science·J HollandS VandePol
Dec 1, 1952·The Journal of Experimental Medicine·J B DANIELSS RICHARDSON

❮ Previous
Next ❯

Citations

Jan 1, 1995·Immunologic Research·E L OleszakC D Platsoucas
Nov 7, 2009·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Ikuo Tsunoda, Robert S Fujinami
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R A GrantJ M Hogle
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·S A HuberM Cunningham
Jan 7, 1998·Immunological Reviews·P MonteyneM Brahic
Aug 1, 1991·Microbial Pathogenesis·M BrahicA McAllister
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·JoAnn P PalmaByung S Kim
Feb 15, 2021·Archives of Virology·J E Libbey, R S Fujinami
Sep 1, 1990·Journal of Virology·A McAllisterM Brahic
May 1, 1994·Journal of Virology·N JarousseA McAllister

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.