Alteration of apoptosis in cleft palate formation and ectomesenchymal stem cells influenced by retinoic acid

Okajimas Folia Anatomica Japonica
F SuwaY R Fang

Abstract

It has been shown that apoptosis is involved in normal embryonic development. The aim of the present study was to elucidate the role and alteration of apoptosis in the pathogenesis of cleft palate induced by retinoic acid (RA) and the ectomesenchymal stem (EMS) cells influenced by RA. RA was administered by gavage to pregnant C57BL/6N strain mice in the experimental group, and the control group received oil alone. Pregnant mice were killed at set periods of time thereafter and histologically analyzed. EMS cells explanted from the palatal shelves of embryonic mice were cultured and characterized by immunohistochemistry, growth curves and population-doubling time. The alterations of apoptosis of EMS cells and developing palatal shelves influenced by RA were evaluated by the terminal deoxynucleotidyl transferase-mediated UTP-biotin nick end-labeling (TUNEL) method. RA-treated mice showed formation of cleft palates resulted from the small size of the palatal shelves and their failure to lift. TUNEL staining showed that the number of apoptotic mesenchymal cells in palatal shelves in the RA-treated mice was increased significantly when compared with the control group. The primary culture of EMS cells proceeded successfully. The popul...Continue Reading

Citations

Jan 15, 2003·Human Reproduction·Fu-Jen HuangYan-Der Hsuuw
Dec 16, 2006·International Journal of Oral and Maxillofacial Surgery·X NieY Jin
Nov 30, 2005·Journal of Anatomy·Sylvia A HilliardYi Ping Chen
Jan 21, 2006·Birth Defects Research. Part C, Embryo Today : Reviews·Irving M ShapiroVickram Srinivas
Oct 27, 2006·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Jing HanYong Li
Apr 13, 2011·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Zhaoyou YaoTingting Zhao
Jul 30, 2020·Birth Defects Research·Yu JiChengji J Zhou

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