PMID: 8592151Feb 1, 1996Paper

Alteration of Ca2+ fluxes in brain microsomes by K+ and Na+: modulation by sulfated polysaccharides and trifluoperazine

Journal of Neurochemistry
J B RochaL de Meis

Abstract

Rat brain microsomes accumulate Ca2+ at the expense of ATP hydrolysis. The rate of transport is not modulated by the monovalent cations K+, Na+, or Li+. Both the Ca2+ uptake and the Ca(2+)-dependent ATPase activity of microsomes are inhibited by the sulfated polysaccharides heparin, fucosylated chondroitin sulfate, and dextran sulfate. Half-maximal inhibition is observed with sulfated polysaccharide concentrations ranging from 0.5 to 8.0 micrograms/ml. The inhibition is antagonized by KCl and NaCl but not by LiCl. As a result, Ca2+ transport by the native vesicles, which in the absence of polysaccharides is not modulated by monovalent cations, becomes highly sensitive to these ions. Trifluoperazine has a dual effect on the Ca2+ pump of brain microsomes. At low concentrations (20-80 microM) it stimulates the rate of Ca2+ influx, and at concentrations > 100 microM if inhibits both the Ca2+ uptake and the ATPase activity. The activation observed at low trifluoperazine concentrations is specific for the brain Ca(2+)-ATPase; for the Ca(2+)-ATPases found in blood platelets and in the sarcoplasmic reticulum of skeletal muscle, trifluoperazine causes only a concentration-dependent inhibition of Ca2+ uptake. Passive Ca2+ efflux from bra...Continue Reading

Citations

Aug 28, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Danielly Cristiny Ferraz da Costa, Ana Maria Landeira-Fernandez
Jun 20, 2003·Journal of Biochemical and Molecular Toxicology·M B MorettoJ B T Rocha
Apr 16, 1998·Biochemical and Biophysical Research Communications·J B RochaL de Meis
Aug 11, 2000·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·A M Landeira-FernandezL de Meis
Oct 19, 2001·The International Journal of Biochemistry & Cell Biology·V P VieiraM R Schetinger
Nov 4, 1996·Brain Research·A J FreitasD O Souza
Nov 23, 2006·The International Journal of Biochemistry & Cell Biology·Carla F FelixHelena M Scofano

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