Alteration of cell adhesion and cell cycle properties of ES cells by an inducible dominant interfering Myb mutant

Oncogene
N IwaiT Nakano

Abstract

The Myb transcription factors, c-Myb, A-Myb, and B-Myb, regulate cell differentiation and/or proliferation. To investigate the role of B-Myb in embryogenesis, we introduced an inducible dominant interfering Myb protein (MERT) into embryonic stem (ES) cells, which express B-Myb as an exclusive member of Myb family. Disruption of normal B-Myb function by the conditional activation of MERT caused a drastic morphological alteration of ES cells and G(1)-S cell cycle arrest. The inhibition of B-Myb function by MERT dissociated tightly packed ES cell colonies into dispersed single cells that subsequently detached from the culture dish. Cell adhesion analyses revealed that suppression of B-Myb function reduced the adhesion with extracellular matrix proteins, such as laminin, collagen, and fibronectin. This reduction was presumably due to decreased cell surface expression of beta1 integrin. Embryoid body formation was also severely retarded by the activation of MERT. This impairment was attributed to reduced expression of E-cadherin, which functions as a homophilic intercellular adhesion molecule. Simultaneously, blocking B-Myb function did not alter the expression of differentiation markers. Our data indicate that B-Myb plays important...Continue Reading

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Citations

Jul 10, 2008·Clinical & Experimental Metastasis·Moyeenuddin SyedGeorg F Weber
Feb 17, 2010·International Journal of Hematology·Shinji Masui
Sep 27, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Elizabeth M LongDouglas A Gray
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Dec 22, 2006·Journal of Cell Science·Kazuya OgawaKohei Miyazono
Jan 12, 2007·Development·Hitoshi Niwa

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