Alteration of cell-cycle regulatory proteins in human oral epithelial cells immortalized by HPV16 E6 and E7

International Journal of Oral and Maxillofacial Surgery
P SdekJ W Zheng

Abstract

E6 and E7 oncoproteins from high-risk human papillomavirus (HPV) can transform cells in tissue culture and induce tumors in vivo by abrogating the cell-cycle checkpoint. To investigate the impact of HPV16 E6 and E7 on the cell-cycle regulatory machinery in oral epithelial cells, normal human oral epithelial cells were transfected with HPV16 E6 and E7 open reading frames, and alterations in cell-cycle regulatory proteins in cells expressing HPV16 E6 and E7 were analyzed. E6 and E7 expression results in immortalization of oral epithelial cells. E7 inactivates retinoblastoma protein (Rb) by forming complexes with hypophosphorylated Rb in immortalized oral epithelial cells. P53 and P21 protein levels were increased in immortalized cells compared to normal primary oral epithelial cells. Cyclin D1-cell-cycle-dependent kinase 4 binary association is disrupted in immortalized oral epithelial cells. These results indicate that E7 plays an important role in abrogation of cell-cycle regulation in oral epithelial cells, with E6 having a smaller impact. This suggests that the pathogenesis of HPV in oral epithelial cells differs from that in cervical epithelial cells.

References

Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·L W ElmoreC C Harris
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·J A Lees, R A Weinberg
Aug 12, 1999·Annals of Medicine·S M Syrjänen, K J Syrjänen
Mar 11, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A S Lundberg, R A Weinberg
Jul 18, 2000·Experimental Cell Research·M K KangN H Park
Dec 11, 2002·Current Opinion in Cell Biology·Olivier Stevaux, Nicholas J Dyson
Jul 2, 2003·Nature Cell Biology·Nicholas B La Thangue

❮ Previous
Next ❯

Citations

May 29, 2013·Experimental and Molecular Pathology·Bin QiaoAlfred K Lam
Feb 17, 2010·Journal of Cancer Research and Clinical Oncology·Lai-ping ZhongZhi-yuan Zhang
Jan 1, 2010·International Journal of Oral and Maxillofacial Surgery·X YangZ-y Zhang
Sep 30, 2008·Archives of Oral Biology·Lai-ping ZhongZhi-yuan Zhang
Jan 15, 2008·Experimental Eye Research·Federico Castro-Muñozledo
Aug 5, 2008·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Lei ZhangZhi-yuan Zhang
Jul 18, 2008·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Seung-Ki MinEun-Cheol Kim
Feb 28, 2013·Journal of Periodontal Research·C E Moffatt-JaureguiR J Lamont
Feb 13, 2010·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Lei ZhangZhiyuan Zhang
Aug 30, 2014·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Christopher T D DickmanCathie Garnis
Aug 2, 2005·International Journal of Oral and Maxillofacial Surgery·J C LeãoS Porter
Nov 6, 2007·American Journal of Otolaryngology·Rosilene Calazans SoaresLeão P Pinto
Jan 7, 2009·Auris, Nasus, Larynx·Márcio Campos OliveiraAntonio de Lisboa Lopes Costa
May 25, 2010·International Journal for Parasitology·T QuackC G Grevelding
Nov 18, 2008·Hematology/oncology Clinics of North America·Laura Vidal, Maura L Gillison
Jul 28, 2007·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Carlos Eduardo Xavier dos Santos Ribeiro da SilvaLuc Louis Maurice Weckx
May 1, 2021·International Journal of Molecular Sciences·Nadezhda A EvtushenkoNadya G Gurskaya

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.