Alteration of cell cycle timing and induction of surface instability in starfish blastomeres microinjected with antibodies to spectrin

Developmental Biology
G K WongD A Begg

Abstract

Spectrin has been implicated in a variety of different processes during late embryogenesis, after transcription of the zygotic genome has been activated. However, relatively little is known about the role of maternally derived spectrin during the early cleavage divisions that give rise to a multicellular embryo. To investigate the role of spectrin in early development, we have microinjected anti-spectrin antibodies into Patiria miniata starfish embryos to inhibit the activity of the maternal pool of spectrin. Microinjection of affinity-purified anti-spectrin antibody, or low to moderate doses of F(ab) fragments, into one blastomere of a two-cell-stage embryo caused a dose-dependent, progressive increase in the length of the cell cycle compared to the uninjected control blastomere. The progeny of injected blastomeres were unable to participate in the formation of a blastula epithelium, instead forming a loose aggregate of cells that eventually stopped dividing. When division stopped, the cells formed surface protrusions and became motile. At high doses of either whole antibody or F(ab) fragments, cells initiated, but failed to complete, cytokinesis. Blastomeres injected with high doses of F(ab) fragments also failed to reform nu...Continue Reading

Citations

Mar 1, 2002·Development, Growth & Differentiation·Tetsuya S Tanaka, Kohji Ikenishi
Aug 19, 2015·Frontiers in Microbiology·Arieh Zaritsky, Conrad L Woldringh
May 14, 2011·Microbiology·Arieh ZaritskyNorbert O E Vischer
Nov 4, 2008·The Journal of Biological Chemistry·Sylvain MetralMarie-Christine Lecomte

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