PMID: 3763404Sep 25, 1986Paper

Alteration of cellular gene expression in adenovirus transformed cells by post-transcriptional mechanisms

Nucleic Acids Research
H T Kao, J R Nevins

Abstract

We have isolated cDNA clones complementary to human mRNAs that are expressed at elevated levels in 293 cells, adenovirus-transformed human embryonic kidney cells, as compared to a normal counterpart of this cell line. Approximately 200 clones out of 100,000 that were screened were positive; 40 of these were isolated, of which 31 were determined to be unique and were further characterized. Each clone detected a mRNA that was 5 to 50 times more abundant in 293 cells than in the non-transformed HEK cell line. For several of these transcripts, the elevated expression appeared to be a function of transformation since they were also high in other human tumor cell lines. Strikingly, we have found that post-transcriptional control is largely responsible for the regulation of the abundance of these mRNAs.

References

Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J L VanniceG M Ringold
Sep 1, 1983·Cell·M R ScottP W Rigby

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Citations

Apr 9, 2008·Physiological Reviews·Rafael LindenRicardo R Brentani
Aug 15, 1997·The Journal of Biological Chemistry·N H BishopricK A Webster

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