Alteration of chemoattractant receptor expression regulates human neutrophil chemotaxis in vivo

Annals of Surgery
Andrew J E SeelyNicolas V Christou

Abstract

To elucidate the mechanisms that regulate human neutrophil delivery in vivo, as well as the mechanisms that lead to observed reduction in polymorphonuclear (PMN) delivery to remote sites in septic patients. Alterations in human PMN chemoattractant receptor expression and chemotactic function in vivo were evaluated in two distinct experiments: exudate PMNs (PMNs that have undergone transmigration to skin window blisters in controls) and septic PMNs (circulating PMNs from septic patients in the intensive care unit) were both separately compared with control circulating PMNs. Exudate PMNs displayed increased C5a receptors and C5a chemotaxis, and reduced interleukin-8 receptors (both IL-8 RA and IL-8 RB) and IL-8 chemotaxis. Septic PMNs displayed reduced C5a and IL-8 receptors and decreased C5a chemotaxis but no change in IL-8 chemotaxis. IL-8 but not C5a receptor gene expression decreased in parallel to receptor alteration. These results suggest that change in PMN chemoattractant receptor expression serves to regulate PMN chemotaxis in vivo; that exudate PMN chemotaxis depends more on C5a than IL-8; and that diminished chemoattractant receptors and chemotaxis in septic PMNs may explain decreased PMN delivery in these patients.

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Citations

Mar 26, 2004·Nature Reviews. Immunology·Peter A Ward
Jan 30, 2008·Immunology and Cell Biology·Hyun LeeCharles R Mackay
Sep 22, 2006·Infection and Immunity·Sabine ZimmermannGertrud Maria Hänsch
Mar 18, 2005·Annual Review of Immunology·Ren-Feng Guo, Peter A Ward
Dec 25, 2004·American Journal of Respiratory and Critical Care Medicine·Michael H TarloweCarl J Hauser
Mar 20, 2012·Critical Care : the Official Journal of the Critical Care Forum·Manu Shankar-HariMervyn Singer
Aug 22, 2003·Critical Care : the Official Journal of the Critical Care Forum·Andrew J E SeelyNicolas V Christou
Apr 11, 2013·Journal of Immunological Methods·Lisa DavidssonJohan Bylund
May 29, 2012·Advanced Drug Delivery Reviews·S Moein MoghimiA Christy Hunter
May 23, 2012·Molecular Immunology·Elizabeth PalmerCarmen W van den Berg
Oct 4, 2016·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Huafeng Zhang, Bingwei Sun
Dec 17, 2003·Shock·Ren-Feng GuoPeter A Ward
Jul 13, 2006·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Michael A FlierlMarkus S Huber-Lang
Aug 5, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ren-Feng GuoPeter A Ward
Apr 3, 2020·Cellular & Molecular Immunology·Mieke MetzemaekersPaul Proost
Jan 22, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carmen W van den BergEamon P McGreal
Jan 7, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Markus Huber-LangPeter A Ward
Aug 7, 2020·International Journal of Molecular Sciences·Dominik JarczakAxel Nierhaus

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