Alteration of D1 and D2 dopaminergic receptor kinetics in specific rat brain regions following repeated administration of opiates

Pharmacology
M A Elwan, M R Soliman

Abstract

The present study was conducted to investigate the effects of repeated administration of opiates on the binding characteristics of D1 and D2 dopamine receptors in specific rat brain regions. Male Sprague-Dawley rats (150-175 g) were adapted for 1 week under controlled conditions (22 +/- 1 degree C, 40% relative humidity, 12 h light:12 h dark illumination cycle). After the adaptation period, rats were randomly assigned into six groups (n = 15/group) for two sets of experiments; one for D1 and the other for D2 receptor binding evaluation. In each set, group 1 served as saline control, group 2 was treated with morphine (1 mg/kg/day for 7 days) and group 3 was treated with naloxone (2 mg/kg/day for 7 days). Following the third day of morphine injection, rats showed restlessness, hyperactivity, increased urination, diarrhea, lacrimation, irritability and squealing on touch, head and body shakes and salivation just prior to morphine dosage. These observed signs are typical for morphine withdrawal. One hour after the last injection, rats were sacrificed by decapitation, brains were removed and kept at -70 degrees C. The frozen brains were further dissected into cortex (CTX), hypothalamus (HYPO), hippocampus (HIPP) and midbrain (MID), ...Continue Reading

Citations

Jan 1, 1996·Peptides·G A OlsonA J Kastin
Apr 2, 1998·General Pharmacology·A O el-Kadi, S I Sharif
Mar 27, 2001·The European Journal of Neuroscience·C L DockstaderD van der Kooy
Jun 19, 2007·Journal of Toxicology and Environmental Health. Part a·Carol M BaldwinMark L Witten
Sep 14, 2010·The Journal of Emergency Medicine·John R RichardsRobert W Derlet
Jan 27, 2015·Behavioural Brain Research·Michael A EmeryShoshana Eitan
May 21, 1999·Journal of Neuroscience Research·M A ElwanN Sakuragawa

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