Alteration of DNA and RNA binding activity of human telomere binding proteins occurs during cellular senescence

Experimental Cell Research
K HubbardH L Ozer

Abstract

The loss of telomere sequences during in vitro and in vivo aging suggests that mechanisms affecting telomere length may have important consequences in cellular senescence. In this study, we have found that the activity of single-stranded telomere binding proteins is increased in nuclear extracts prepared from senescent human diploid fibroblasts compared to actively growing cells. Since single-stranded telomere binding proteins are closely related to RNA binding proteins, we examined the binding activity of several major RNA binding proteins to RNA by uv cross-linking. The level of activity was greatly diminished and the overall pattern of uv cross-linked products were altered in extracts prepared from senescent cells. Furthermore, Western analysis revealed a concurrent decrease in senescent extracts of the protein level for many RNA binding proteins, including those which bind to telomere sequence. Although the reduction in the level of these proteins parallels the reduced activity in RNA binding, the paradoxical increased telomere binding activity exhibited by extracts from older cells suggests a complex relationship between these proteins with RNA and DNA. Moreover, the reduced RNA binding activity of these proteins indicates...Continue Reading

Citations

Oct 12, 2010·Nucleic Acids Research·Andrew M ParrottMichael B Mathews
Jun 15, 2011·The Journal of Clinical Investigation·Kan CaoFrancis S Collins
Nov 7, 2015·Journal of Molecular Neuroscience : MN·Valerie LeducJudes Poirier
Sep 1, 1996·Journal of Cellular Physiology·M B SmallK A Sethi
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·K WheatonK Riabowol
Jan 31, 1997·The Journal of Biological Chemistry·L TomáskaH Fukuhara
Jan 1, 1996·Experimental Gerontology·H L OzerP C Patsalis

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