Alteration of gene expression by intestinal epithelial cells precedes colitis in interleukin-2-deficient mice

The American Journal of Physiology
M A MeijssenD K Podolsky

Abstract

Intestinal epithelial cells may be actively involved in the immunoregulatory pathways leading to intestinal inflammation. The aim of this study was to assess expression by intestinal epithelial cells of cytokines with potential involvement in the development of intestinal inflammation in interleukin (IL)-2-deficient [(-/-)] mice. Wild-type mice, mice heterozygous for the disrupted IL-2 gene, and IL-2(-/-) mice were studied at 6, 16, and 24 wk of age. The mRNA levels of transforming growth factor-beta 1 (TGF-beta 1), tumor necrosis factor-alpha (TNF-alpha), IL-1 beta, IL-6, IL-15, KC, JE, and CD14 in colonic and small intestinal epithelial cells were assessed by Northern blot analysis. CD14 was also measured by Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR). TGF-beta 1 mRNA was constitutively expressed in both colonic and small intestinal epithelial cells with increased expression in the colonic epithelium of colitic mice. CD14 was detected only in colonic epithelial cells, and mRNA levels increased severalfold in IL-2(-/-) mice with colitis. Northern analysis demonstrated increased levels of TGF-beta 1 and CD14 mRNA in colonic epithelial cells of IL-2(-/-) mice before the development of signs of c...Continue Reading

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Citations

Mar 1, 2012·World Journal of Gastrointestinal Pathophysiology·Linda Chia-Hui YuYen-Hsuan Ni
Sep 26, 2014·World Journal of Gastroenterology : WJG·Rima RamonaiteLimas Kupcinskas
Jun 15, 2006·World Journal of Gastroenterology : WJG·Christian-U RiedelStephanie Blum
Mar 17, 2010·Applied and Environmental Microbiology·Julia PreisingChristian U Riedel
Mar 15, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Linda C H YuMary H Perdue
Oct 7, 2006·Current Opinion in Gastroenterology·A K BhanA Mizoguchi
Sep 22, 2005·Immunology and Cell Biology·Hubertus P A Jersmann

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