Alteration of glial cell line-derived neurotrophic factor family receptor alpha-2 mRNA expression and its co-expression with neuronal nitric oxide synthase in pelvic ganglia following unilateral cavernous nerve injury

International Journal of Urology : Official Journal of the Japanese Urological Association
Shin-Ichi HisasueT Tsukamoto

Abstract

The goal of this study was to determine the alterations of glial cell line-derived neurotrophic factor family receptor alpha-2 (GFRalpha2) mRNA expression in the major pelvic ganglia (MPG) and their relationship to the marker for the neural plasticity (growth-associated protein 43: GAP-43) and neuronal nitric oxide synthase (nNOS)-positive neurons following cavernous nerve injury. Cavernous nerves were transected unilaterally in 24 Sprague-Dawley rats aged 8 weeks. We used nine sham operated same animals as controls. Bilateral MPGs were harvested at 1, 3, and 6 months following nerve injury. The GFRalpha2 and GAP-43 mRNA expressions of the sham group and the injury group (3 months after surgery) were investigated by reverse transcription-polymerase chain reaction. We also investigated the expression profile of GFRalpha2 mRNA by in situ hybridization combined with nNOS immunostaining. It was revealed semi-quantitatively that the GAP43 mRNA expression moderately increased in the intact MPG, and GFRalpha2 mRNA was maintained in the intact MPG but not in the injured one. A histological double-labeling study showed that the number of GFRalpha2 mRNA- and nNOS-positive neurons increased in the intact MPG and most GFRalpha2 mRNA expres...Continue Reading

References

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Jun 30, 2005·International Journal of Urology : Official Journal of the Japanese Urological Association·Takashi ShimizuTaiji Tsukamoto
Aug 17, 2005·The European Journal of Neuroscience·Yewlan Wanigasekara, Janet R Keast
Sep 6, 2005·Neurobiology of Aging·Shin-ichi HisasueTaiji Tsukamoto

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Citations

Sep 29, 2009·International Journal of Urology : Official Journal of the Japanese Urological Association·Shinichi YamashitaTaiji Tsukamoto
Mar 21, 2017·BioMed Research International·Laura G Hernández-AragónFrancisco Castelán

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