Abstract
Interleukin-6 (IL-6) is a principal proinflammatory cytokine inducing the acute phase response in various tissues, including liver. Here, we adopt the FD-LC-MS/MS method, consisting of fluorogenic derivatization (FD), separation by liquid chromatography (LC), and identification of proteins by LC-tandem mass spectrometry (MS/MS), to reveal how exposure to IL-6 alters temporally the intracellular secretory acute phase response (sAPR) proteins expressed in human hepatocytes as compared to non-exposure. Nine altered sAPR proteins were identified in cultures in response to IL-6. Seven of them (serum amyloid A protein, haptoglobin, fibrinogen α chain, fibrinogen β chain, fibrinogen γ chain, α(1)-acid glycoprotein and α(1)-antitrypsin) were significantly increased and two (β(2)-glycoprotein 1 and transferrin) were significantly decreased in response to IL-6. In addition, the transmission speed of transferrin might be much faster than the other sAPR proteins. These results suggest a different molecular mechanism for protein synthesis and the secretory pathway among the sAPR proteins. In this study, we observed the simultaneously and temporally altered expression of sAPR proteins which had been induced by exposure to IL-6 in human hepat...Continue Reading
References
Dec 15, 1991·Gene·H MehdiM E Peeples
May 23, 1988·FEBS Letters·J V CastellP C Heinrich
Aug 30, 1988·Biochemical and Biophysical Research Communications·H J MoshageS H Yap
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F YangB H Bowman
Mar 15, 1983·The Biochemical Journal·S S MacIntyreI Kushner
Feb 1, 1994·Immunology Today·H Baumann, J Gauldie
Nov 1, 2000·Metabolic Engineering·A JayaramanC M Roth
Dec 25, 2003·European Journal of Human Genetics : EJHG·Damian C CrowtherDavid A Lomas
Mar 2, 2005·Tissue Engineering·Arul JayaramanCharles M Roth
Sep 22, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Cédric CoulouarnJean-Philippe Salier
Feb 18, 2006·Cytokine·Emilia BonarAleksander Koj
Oct 28, 2008·Biochemical and Biophysical Research Communications·Megumi NarisadaEiji Miyoshi
Aug 15, 2009·Journal of Proteome Research·Astrid SlanyChristopher Gerner
Dec 2, 2010·Biomedical Chromatography : BMC·Kazuhiro ImaiKatsunori Nakata
Citations
Feb 28, 2015·Biological Research·Gustavo OrtizJuan E Gallo
Jun 24, 2014·Journal of Pharmaceutical and Biomedical Analysis·Tomoko Ichibangase, Kazuhiro Imai
Jul 3, 2013·Biomedical Chromatography : BMC·Tomoko IchibangaseKazuhiro Imai
May 28, 2014·Biomedical Chromatography : BMC·Tomoko IchibangaseKazuhiro Imai
May 28, 2014·Biomedical Chromatography : BMC·Katsunori NakataKazuhiro Imai
Feb 18, 2015·Proteomics·Julien BertrandMoïse Coëffier
May 17, 2015·Genomics·Samuel W LukowskiMarguerite Neerman-Arbez
Aug 14, 2015·International Journal of Toxicology·Martin D Green
Nov 25, 2014·The Analyst·Katsunori NakataKazuhiro Imai
Sep 14, 2017·Journal of Assisted Reproduction and Genetics·Laura M L CarvalhoKarina B Gomes