Alteration of intracellular secretory acute phase response proteins expressed in human hepatocyte induced by exposure with interleukin-6

Cytokine
Katsunori NakataKazuhiro Imai

Abstract

Interleukin-6 (IL-6) is a principal proinflammatory cytokine inducing the acute phase response in various tissues, including liver. Here, we adopt the FD-LC-MS/MS method, consisting of fluorogenic derivatization (FD), separation by liquid chromatography (LC), and identification of proteins by LC-tandem mass spectrometry (MS/MS), to reveal how exposure to IL-6 alters temporally the intracellular secretory acute phase response (sAPR) proteins expressed in human hepatocytes as compared to non-exposure. Nine altered sAPR proteins were identified in cultures in response to IL-6. Seven of them (serum amyloid A protein, haptoglobin, fibrinogen α chain, fibrinogen β chain, fibrinogen γ chain, α(1)-acid glycoprotein and α(1)-antitrypsin) were significantly increased and two (β(2)-glycoprotein 1 and transferrin) were significantly decreased in response to IL-6. In addition, the transmission speed of transferrin might be much faster than the other sAPR proteins. These results suggest a different molecular mechanism for protein synthesis and the secretory pathway among the sAPR proteins. In this study, we observed the simultaneously and temporally altered expression of sAPR proteins which had been induced by exposure to IL-6 in human hepat...Continue Reading

References

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Dec 2, 2010·Biomedical Chromatography : BMC·Kazuhiro ImaiKatsunori Nakata

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Citations

Feb 28, 2015·Biological Research·Gustavo OrtizJuan E Gallo
Jun 24, 2014·Journal of Pharmaceutical and Biomedical Analysis·Tomoko Ichibangase, Kazuhiro Imai
May 28, 2014·Biomedical Chromatography : BMC·Katsunori NakataKazuhiro Imai
May 17, 2015·Genomics·Samuel W LukowskiMarguerite Neerman-Arbez
Nov 25, 2014·The Analyst·Katsunori NakataKazuhiro Imai
Sep 14, 2017·Journal of Assisted Reproduction and Genetics·Laura M L CarvalhoKarina B Gomes

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