Alteration of macrophage responsiveness to platelet-activating factor by interferon-gamma and lipopolysaccharide

Cellular Immunology
A D Howard, K L Erickson

Abstract

Platelet-activating factor (PAF) can modulate several macrophage responses associated with tumoricidal and inflammatory activity. To determine how macrophage responsiveness to PAF may be altered by interferon-gamma (IFN-gamma) or lipopolysaccharide (LPS), we studied PAF receptor-associated activities. Pretreatment of murine peritoneal macrophages with either LPS or IFN-gamma suppressed macrophage responsiveness to both PAF-induced calcium mobilization and superoxide anion (O2-) production. This suppression of macrophage responsiveness to PAF was maximal when 25 U/ml IFN-gamma or 100 ng/ml LPS was initially added for 6 hr. Macrophages pretreated with LPS or IFN-gamma remained refractory to PAF-induced rise in intracellular calcium for 4 to 24 hr. Macrophages preincubated with 25 U/ml IFN-gamma remained refractory to PAF-induced calcium mobilization for up to 4 hr. LPS and IFN-gamma treatment also decreased PAF-induced, calcium-dependent O2- production. When added together, IFN-gamma increased the suppression of PAF-induced intracellular calcium mobilization and inhibited O2- production mediated by LPS. To assess whether suppression was mediated through altered PAF receptors, binding affinities were determined; two binding affini...Continue Reading

Citations

Nov 6, 2010·Clinical Endocrinology·Matthew Blatnik, Catherine I Soderstrom
Oct 18, 2011·Lasers in Medical Science·Tsui Hsien HuangChia Tze Kao
Mar 3, 1998·Nutrition Reviews·K L Erickson

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