Alteration of microRNA profiling in sphere-cultured ovarian carcinoma cells

Oncology Letters
Hwa Jun ChaJae Ho Lee

Abstract

Ovarian cancer is an aggressive and lethal cancer, which in part, can be attributed to complications in the effective detection of this disease during early stages of progression. Frequently, epithelial ovarian cancer is disseminated to the abdominal cavity and forms multicellular aggregates. This unique early metastatic event, and formation of the multicellular aggregate is implicated to provide a basis for understanding the underlying molecular mechanisms of metastasis in ovarian cancer. Therefore, a 3-dimensional (3D) sphere culture system was established in the present study to mimic the later stages of ovarian cancer. The aim of the present study was to investigate whether microRNAs (miRNAs), which have functions in metastasis and chemoresistance in various cancer models, are altered in ovarian cancer cells by 3-dimensional (3D) culture. A multicellular aggregate of SKOV3ip1 ovarian carcinoma cells was generated using a 3D sphere culture system. Cell viability analysis demonstrated that the sphere-cultured SKOV3ip1 cells exhibited chemoresistance compared with those in a conventional 2-dimensional (2D) monolayer cultured SKOV3ip1 system. Under the same experimental conditions, 71 upregulated miRNAs and 63 downregulated miR...Continue Reading

References

Jul 12, 2002·Nature·Gregory J Hannon
Mar 27, 2003·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·G CormioL Selvaggi
Feb 5, 2004·British Journal of Cancer·H Jin, J Varner
Dec 14, 2004·The New England Journal of Medicine·Stephen A Cannistra
May 3, 2005·Nature Reviews. Cancer·Honami Naora, Denise J Montell
Jul 8, 2005·Molecular Biology of the Cell·Nathan D GallantAndrés J García
Oct 24, 2006·The Journal of Surgical Research·Yupei ZhaoPo Sing Leung
Jan 27, 2007·The American Journal of Surgical Pathology·David W KindelbergerChristopher P Crum
May 9, 2008·RNA·Dominic Didiano, Oliver Hobert
Sep 26, 2009·Endocrine-related Cancer·Benjamin BoyerinasMarcus E Peter
Oct 13, 2009·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Shozo YoshidaHiroshi Kobayashi
Jan 26, 2010·Nature Reviews. Immunology·Ryan M O'ConnellDavid Baltimore
Mar 30, 2010·Journal of Cellular and Molecular Medicine·Shuang-Di LiXiao-Ping Wan
Jul 24, 2010·The American Journal of Pathology·Ernst Lengyel
May 3, 2011·Current Genomics·Leigh-Ann Macfarlane, Paul R Murphy
Jul 15, 2011·Advances in Experimental Medicine and Biology·Nicolas J Lehrbach, Eric A Miska
Jul 16, 2011·Hormones & Cancer·Antonio F SaadAnil K Sood
Sep 29, 2011·Clinical Epigenetics·Kristy R KutanziIgor P Pogribny
Jan 18, 2012·Journal of Experimental & Clinical Cancer Research : CR·Ning WangXingsheng Yang
Feb 20, 2014·Journal of Tissue Engineering·Guojie XuJinming Zhao
Mar 7, 2014·Frontiers in Genetics·Sarah M PetersonClare Bates Congdon
Jan 28, 2015·Neoplasia : an International Journal for Oncology Research·Louis-Bastien WeiswaldVirginie Dangles-Marie
Mar 1, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Divya LenkalaR Stephanie Huang
May 18, 2016·Journal of Ovarian Research·J R RibeiroR G Moore

❮ Previous
Next ❯

Citations

Feb 15, 2020·Asian-Australasian Journal of Animal Sciences·Zhuandi GongSuocheng Wei

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.