Alteration of Na,K-ATPase isoenzymes in diabetic cardiomyopathy: effect of dietary supplementation with fish oil (n-3 fatty acids) in rats

Diabetologia
A GerbiJ M Maixent

Abstract

Diabetic cardiomyopathy has been associated with a decrease in Na,K-ATPase activity and expression, as well as alterations in membrane lipid composition. The aim of this study was twofold: 1) to document in rats the effect of streptozotocin-induced diabetes on myocardial Na,K-ATPase and fatty acids, and 2) to evaluate the potential effect of a dietary supplementation with fish oil (n-3 fatty acids) on the streptozotocin-induced changes. Assays were performed in purified cardiac plasma membranes to determine Na,K-ATPase activity, expression of the different alpha- and beta-subunits of Na,K-ATPase, and the fatty acid content of total phospholipids. Relative abundance of the mRNAs encoding the alpha 1, alpha 2 and beta 1 isoforms was studied by Northern blot analysis. Results demonstrated that diabetes significantly decreased activities of alpha 1 and alpha 2 isoforms and mRNA levels of alpha 2 and beta 1 isoforms, and, at the protein level, increased alpha 1-isoforms and decreased both alpha 2- and beta 1-isoforms. Changes in fatty acid content of the membrane were consistent with inhibition of desaturase. Fish-oil supplementation produced an increase in membrane incorporation of eicosapentaenoic acid. It also increased the level...Continue Reading

Citations

Jun 17, 2008·Molecular and Cellular Biochemistry·Jérôme KalifaMonique Bernard
Mar 23, 2000·European Journal of Biochemistry·S SennouneJ M Maixent
Mar 28, 2009·Pharmacological Reviews·Alexei Y BagrovOlga V Fedorova
Feb 26, 2013·Heart Failure Reviews·Naranjan S DhallaVijayan Elimban
May 16, 2000·Journal of Cardiovascular Electrophysiology·J M MaixentS Lévy
Feb 23, 2013·The Journal of Biological Chemistry·Haim HavivSteven J D Karlish
Apr 16, 1998·Journal of Hypertension·V MayolJ M Maixent
Dec 31, 2002·Journal of Receptor and Signal Transduction Research·Jean-Michel MaixentSamuel Lévy
Aug 23, 2000·Medicina clínica·A Fernández-FúnezA Hernández
Feb 10, 2007·Medical Hypotheses·Zorica ZakulaEsma R Isenovic
Sep 28, 2005·Journal of Cellular Biochemistry·Irving G JoshuaSuresh C Tyagi
Jul 8, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Marie A McAnuffHelen N Asemota
Apr 3, 2008·Experimental Diabetes Research·Claire E Hills, Nigel J Brunskill
Aug 2, 2008·Experimental Diabetes Research·T ForstA Pfützner
May 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S SennouneJ M Maixent
Oct 6, 2006·American Journal of Physiology. Cell Physiology·Peter S HansenHelge H Rasmussen
Aug 16, 2019·Journal of Cardiovascular Pharmacology·Olga V FedorovaAlexei Y Bagrov
Dec 22, 1999·The American Journal of Physiology·J M MaixentM Bernard
Feb 26, 2020·European Psychiatry : the Journal of the Association of European Psychiatrists·Gerwyn MorrisBasant K Puri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Diseases: Risk Factors

Cardiovascular disease is a significant health concern. Risk factors include hypertension, obesity, dyslipidemia and smoking. Women who are postmenopausal are at an increased risk of heart disease. Here is the latest research for risk factors of cardiovascular disease.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.