Alteration of OBCAM conformation as a result of opioid receptor expression and opioid ligand treatment

Brain Research
C M LaneN M Lee

Abstract

Several lines of evidence link the opioid binding cell adhesion molecule (OBCAM) to opioid function. When delta-opioid receptor cDNA (DOR-1) was expressed in CHO cells, OBCAM immunoreactivity (OBCAM-ir) was detected. Transfected cell lines which displayed high opioid binding also expressed high cell surface OBCAM-ir, while untransfected CHO and vector control cells did not. The positive control, neural cell adhesion molecule (NCAM), a protein with structural homology to OBCAM, displayed the same levels of immunofluorescence in transfected and nontransfected cell lines. Membranes from CHO cells transfected with and expressing a variety of muscarinic and dopamine receptors were tested for immunoreactivity. No significant OBCAM-ir was detected in any of these cell membranes. When anti-OBCAM peptide antibodies were used for immunoblots of CHO cells, untransfected, non-binding transfected, and high binding transfected cells revealed the same banding patterns with approximately equal intensity. These observations suggest that in untransfected cells OBCAM is either not present on the extracellular side of the CHO cell membrane or that it exists in an altered conformation which changes upon transfection with opioid receptors to allow r...Continue Reading

References

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C M LaneN M Lee
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B L KiefferC G Hirth
Dec 28, 1992·Science·C J EvansR H Edwards
Feb 1, 1990·Journal of Protein Chemistry·C S WuJ T Yang
Oct 1, 1987·Journal of Neurochemistry·J HasegawaN M Lee
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T M ChoH H Loh
Jul 29, 1988·Biochemical and Biophysical Research Communications·S RoyN M Lee
Apr 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Hassell, A R Hand
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G Koski, W A Klee
Dec 17, 1982·Journal of Immunological Methods·G D JohnsonE J Holborow
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K YasudaG I Bell

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Citations

Feb 2, 1999·The British Journal of Psychiatry : the Journal of Mental Science·D Curtis

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