Alteration of seminal proteins during freeze-drying of bovine semen

Journal of Dairy Science
R S JeyendranE F Graham

Abstract

Bovine semen in TEST-yolk extender was frozen, freeze-dried to 50, 25, 12, 6, and less than 2% residual moisture, and stored at -196 degrees C. The freeze-dried semen was rehydrated, sampled for protein analysis, and used to inseminate cattle. Agar gel electrophoresis revealed no moisture was reduced to less than 6%. At that point, the percent of cationic proteins and neural proteins decreased with a concurrent increase in anionic migrating proteins. Immunodiffusion data with antisera against spermatozoa and seminal plasma revealed no difference in formation of precipitin lines if residual moisture was at least 6%. However, semen freeze-dried to 2% residual moisture was modified antigenically as certain precipitin lines were lost, new lines appeared, and concentration of other seminal antigens decreased. The absence of fertility with semen freeze-dried to 2% residual moisture is hypothesized to 2% residual moisture is hypothesized to be from alteration of the tertiary structure of certain essential seminal proteins.

References

Jan 1, 1974·Advances in Protein Chemistry·I D Kuntz, W Kauzmann
Mar 1, 1972·Journal of Dairy Science·E F GrahamK I Brown
Jan 27, 1971·Journal of the American Chemical Society·I D Kuntz
Jun 1, 1981·Cryobiology·R S JeyendranM K Schmehl
Apr 13, 1960·Annals of the New York Academy of Sciences·H T MERYMAN
Feb 1, 1963·Journal of Reproduction and Fertility·H T MERYMAN, E KAFIG
Dec 9, 1961·Nature·R G SAACKE, J O ALMQUIST

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Citations

May 17, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Yosuke KawaseHiroshi Suzuki
Jul 14, 1998·Nature Biotechnology·T Wakayama, R Yanagimachi
Dec 1, 1989·Journal of Dairy Science·A G Hunter
May 10, 2011·The Journal of Reproduction and Development·Yosuke Kawase, Hiroshi Suzuki
Aug 5, 2000·Animal Reproduction Science·R Vishwanath, P Shannon

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