PMID: 9432028Jan 31, 1998Paper

Alterations in Bcl-2/Bax protein levels in platelets form part of an ionomycin-induced process that resembles apoptosis

British Journal of Haematology
D M VanagsM Aguilar-Santelises

Abstract

Platelets are physiologically anucleated cells, derived from megakaryocytes, that undergo vesiculation and transformation into small particles when they are stimulated in vitro by ionomycin and other agents. Electron microscopy images suggest a similarity to apoptosis in cells with nuclei, which ends with cell disintegration and formation of apoptotic bodies. By PCR, we have demonstrated mRNA expression of bcl-2, bax, and p53 in highly purified non-stimulated platelets. A side-scatter shift and a decrease in the Bcl-2/Bax protein ratio were observed by flow cytometry analysis after stimulation with ionomycin. The ionomycin-induced modifications were inhibited by the calpain I inhibitor calpeptin and, less effectively, by VAD-cmk, a broad-spectrum caspase inhibitor. However, caspase 3-like activity was very low, with only a twofold increase after ionomycin stimulation, as measured by the cleavage of the fluorogenic peptide substrate DEVD-AMC. Our data indicate that platelets may constitute a natural model for the analysis of cytoplasmic events in apoptosis.

Citations

Jul 4, 2012·Apoptosis : an International Journal on Programmed Cell Death·Syeda T TowhidFlorian Lang
Mar 3, 2012·Journal of Thrombosis and Thrombolysis·Armen V GyulkhandanyanValery Leytin
May 3, 2003·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Valery Leytin, John Freedman
Jan 6, 2007·Cell Death and Differentiation·H ZhangC Tse
Jan 19, 2013·The Journal of Biological Chemistry·Manasa K NayakDebabrata Dash
Sep 11, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Sten OrreniusBoris Zhivotovsky
Jul 7, 2011·International Journal of Molecular Sciences·Weilin ZhangKesheng Dai
Oct 27, 2004·Intensive Care Medicine·Jose IrazuztaBasilia Zingarelli
Nov 8, 2011·Blood Reviews·Valery Leytin
Jul 14, 2010·Seminars in Hematology·Michael J White, Benjamin T Kile
Feb 23, 2010·Thrombosis Research·Zhicheng WangKesheng Dai
Jan 19, 2010·Biochemical and Biophysical Research Communications·Elisabetta StrafaceDomenico Del Principe
Mar 29, 2003·Transfusion·Peter L PerrottaEdward L Snyder
Jul 28, 2009·Journal of Thrombosis and Haemostasis : JTH·B T Kile
Jun 26, 2003·Transfusion·A M BertinoD J Kuter
Jul 31, 2008·Journal of Thrombosis and Haemostasis : JTH·J J LopezJ A Rosado
Sep 12, 2006·Journal of Thrombosis and Haemostasis : JTH·V LeytinJ Freedman
Sep 15, 2006·Journal of Thrombosis and Haemostasis : JTH·G L Dale, P Friese
Feb 13, 2013·British Journal of Haematology·Armen V GyulkhandanyanValery Leytin
Aug 26, 2010·Journal of Thrombosis and Haemostasis : JTH·D E van der WalJ W N Akkerman
Jan 29, 2014·British Journal of Haematology·Benjamin T Kile
Oct 16, 2015·Scientific Reports·Somanathapura K NaveenKumarKanchugarakoppal S Rangappa
Jun 29, 2004·Biochemical and Biophysical Research Communications·Valery LeytinJohn Freedman
Jan 10, 2002·Redox Report : Communications in Free Radical Research·M G Ormerod
Jan 30, 2013·Biochemical Society Transactions·Sergey Zharikov, Sruti Shiva
Jun 26, 2015·Journal of Cancer Research and Clinical Oncology·Rui-Yan ZhangDong-Sheng Pei
Feb 27, 2014·The International Journal of Biochemistry & Cell Biology·Luiz F Garcia-Souza, Marcus F Oliveira
May 17, 2013·Seminars in Hematology·Stephan von GuntenHans-Uwe Simon
Apr 11, 2013·Methods : a Companion to Methods in Enzymology·Sandra PoschAndreas Ebner
Apr 22, 2016·Platelets·Marion Lebois, Emma C Josefsson
Mar 27, 2007·Cell·Kylie D MasonBenjamin T Kile

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