Alterations in constituent urinary proteins in response to bladder outlet obstruction in rats

The Journal of Urology
J A BurnsW A See

Abstract

Benign prostatic hyperplasia, resulting in bladder outflow obstruction, induces well recognized clinical symptoms and morphologic bladder changes. Despite these phenomenon, relatively little is known with regard to the precise molecular events occurring in the bladder as a consequence of obstruction. In an effort to screen for alterations in bladder gene expression induced by obstruction, and/or alterations in uroepithelial integrity, this study compared pre- and post-obstructive constituent urinary proteins in an animal model. Outlet obstruction was created using a previously established model system. Experimental animals were surgically obstructed for either 2 or 7 days, at which time the urine was aspirated and the bladders removed and weighed. Urinary proteins were separated using 2-D PAGE. Following comparison of sham versus experimental animals, microsequencing was performed on proteins that were down regulated. Duplicate experiments confirmed the presence of outflow obstruction. Statistically significant increases (p <0.01) in bladder weights were seen at 2 and 7 days in the obstructed groups as compared with both sham and control groups. 2-D PAGE demonstrated a down regulation of three urinary proteins post-obstruction....Continue Reading

References

Jun 13, 1989·Biochemistry·J M BradyR J MacDonald
Jan 1, 1987·Methods in Enzymology·K OgataE M Tan
Aug 13, 1985·Biochemistry·P L Ashley, R J MacDonald
Dec 1, 1973·British Journal of Urology·R T WarwickJ Weir
Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris
Sep 1, 1984·The Journal of Urology·B Uvelius, A Mattiasson
Feb 1, 1994·British Journal of Urology·C R Chapple, D Smith
Jan 1, 1995·Electrophoresis·M R BüelerD J Vonderschmitt
Jan 1, 1994·World Journal of Urology·R M LevinP A Longhurst
Dec 1, 1993·Forensic Science International : Synergy·S D Ferrara
Jan 1, 1995·Advances in Experimental Medicine and Biology·M C DupontW D Steers
Apr 1, 1996·The Journal of Urology·A J Bueschen
Apr 1, 1997·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·J Barkin
Nov 1, 1993·Trends in Cardiovascular Medicine·N Ferrara

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Citations

May 15, 2021·Scientific Reports·Michelle von SiebenthalKatia Monastyrskaya

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