Alterations in cytosine methylation and species-specific transcription induced by interspecific hybridization between Oryza sativa and O. officinalis.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Huajun JinGuangcun He

Abstract

Interspecific hybridization and polyploidization may involve programmed genetic and epigenetic changes. In this study, we used the methylation-sensitive amplified polymorphism (MSAP) method to survey cytosine methylation alterations that occurred in F(1) hybrid and BC(1) progeny following interspecific hybridization between Oryza sativa and O. officinalis. Across all 316 parental methylated sites, 25 (7.9%) cytosine methylation alterations were detected in the F(1) and/or BC(1) progeny. Thirty additional cytosine methylation alterations were detected at parental non-methylated or novel sites. In total, 55 cytosine methylation alterations (90.9% of all alterations) were detected in the F(1) hybrid, which were maintained in the BC(1) progeny. The alterations in cytosine methylation were biased toward the O. officinalis parent and were in some cases repeatable in independent hybridizations between O. sativa and O. officinalis. Twelve fragments showing cytosine methylation alterations were isolated, sequenced and subsequently validated by methylation-sensitive Southern blot analysis. Where possible, we designed species-specific primers to amplify the polymorphic transcripts from either the O. sativa or the O. officinalis parent usi...Continue Reading

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Citations

Feb 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ian K GreavesElizabeth S Dennis
May 28, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Guangming HeXing Wang Deng
Jul 17, 2014·Journal of Applied Genetics·Donghui FuMin Zhang
Jan 26, 2017·Scientific Reports·Meiling ZouWenquan Wang
Jan 12, 2020·Plant Biotechnology Journal·Pallavi SinhaRajeev K Varshney
Apr 24, 2021·Transgenic Research·S Selma, D Orzáez
Apr 5, 2011·Biochimica Et Biophysica Acta·Michael GroszmannW James Peacock

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