Alterations in cytoskeletal protein sulfhydryls and cellular glutathione in cultured cells exposed to cadmium and nickel ions

Toxicology
W LiI N Chou

Abstract

To understand the mechanisms of Cd2+ and Ni2+ cytotoxicity, we have studied the effects of these two metal ions on the organization of cytoskeletal elements, microtubules (MT) and microfilaments (MF), cytoskeletal protein sulfhydryls and cellular glutathione (GSH) in cultured 3T3 cells. At a metal ion dose that caused 95% inhibition of DNA synthesis, Cd2+ (10 microM, 16 h exposure) induced MT depolymerization whereas Ni2+ (2 mM, 20 h exposure) elicited MT aggregation and bundling. Under these conditions, Cd2+ and Ni2+ also caused MF aggregation and redistribution. Furthermore, exposure of cells to Cd2+ resulted in a dose-dependent increase in cytoskeletal protein sulfhydryls and cellular GSH levels. In contrast, treatment of cells with Ni2+ resulted in a dose-dependent decrease in cytoskeletal protein sulfhydryls as well as cellular GSH content. Time course studies showed that cells exposed to 10 microM Cd2+ exhibited a biphasic response in regulating their cytoskeletal protein sulfhydryls and cellular GSH, e.g. an initial decrease followed by a steady recovery and overshooting upon prolonged incubation. However, restoration of cytoskeletal protein sulfhydryls occurred approximately 2 h after commencement of cellular GSH recove...Continue Reading

References

Feb 1, 1979·Environmental Health Perspectives·R J Cousins
May 1, 1992·Toxicology and Applied Pharmacology·W Li, I N Chou
Nov 1, 1990·Toxicology and Applied Pharmacology·K C Lin, I N Chou
Sep 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R K SinghalA Meister
Jul 1, 1988·Archives of Biochemistry and Biophysics·F MirabelliS Orrenius
Jul 1, 1986·Cell Biology International Reports·B A Perrino, I N Chou
Dec 1, 1984·Archives of Biochemistry and Biophysics·D Di MonteS Orrenius
Apr 10, 1984·Biochemistry·H FaulstichC Stournaras
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E KrauhsH Ponstingl
Jan 1, 1989·Toxicology in Vitro : an International Journal Published in Association with BIBRA·B A Perrino, I N Chou

❮ Previous
Next ❯

Citations

Feb 1, 1994·Cell Biology and Toxicology·M Sugiyama
Nov 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S H YoshidaM E Gershwin
Feb 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J C YalowichV E Kagan
Feb 1, 1995·Free Radical Biology & Medicine·S J Stohs, D Bagchi
Dec 4, 2003·Mutation Research·Kazimierz S KasprzakKonstantin Salnikow
Dec 3, 2003·Toxicology and Applied Pharmacology·Wande LiIih-Nan Chou
Dec 5, 1998·Chemosphere·M CamatiniC Urani
Sep 3, 2002·Toxicology·Elisabetta CasalinoClemente Landriscina
Jan 8, 1999·Biochimica Et Biophysica Acta·I DalleDonneR Colombo
Jun 7, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·O J MesnaR A Andersen
Mar 30, 2002·Critical Reviews in Oncology/hematology·Dimitrios Galaris, Angelos Evangelou
Mar 30, 2002·Critical Reviews in Oncology/hematology·E Denkhaus, K Salnikow
Aug 8, 2001·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·E HermeszJ Nemcsók
Aug 8, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Patrick J KennedyPaul Russell
Aug 22, 2007·Molecular and Cellular Biology·Angelo G ScibettaJoyce Taylor-Papadimitriou
Nov 12, 2002·Environmental Health Perspectives·Konstantin SalnikowMax Costa
Apr 4, 2012·International Journal of Environmental Research and Public Health·Yinzhi ZhaoWande Li
Oct 18, 2008·Reproductive Toxicology·Prasenjit MannaParames C Sil
Apr 29, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Pavel PribylVilém Zachleder
Dec 25, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Guang-Yong LiByung-Hoon Lee
Oct 27, 2004·Chemico-biological Interactions·Pei-Ming YangLih-Yuan Lin
Sep 1, 2006·Environmental Toxicology and Pharmacology·Branka I OgnjanovićZorica S Saičić
Jul 1, 2006·Environmental Toxicology and Pharmacology·Katharine M W Haberstroh, Carolyn M Kapron
Jun 14, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jinxiang WangLan Wang
Jan 9, 2004·Acta Biologica Hungarica·K Said AliEdit Hermesz
May 1, 1997·Toxicology and Applied Pharmacology·S LynnK Y Jan
Oct 3, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kahkashan RashidParames C Sil
Dec 18, 2009·Nutrition Research and Practice·You Jin KimMi Kyung Kim
Nov 29, 2005·Toxicology Letters·Aleksandra Witkiewicz-Kucharczyk, Wojciech Bal
May 1, 2009·Environmental Toxicology and Pharmacology·Rolf Gebhardt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.