Alterations in differentiation and pyrimidine pathway enzymes in 5-azacytidine resistant variants of a myoblast line

Journal of Cellular Physiology
S K NgB D Sanwal

Abstract

5-azacytidine at concentrations higher than 5 muM inhibited the differentiation of a rat myoblast line in vitro. It was also somewhat cytotoxic at this level. Variants resistant to the cytotoxic effect of 5-azacytidine were obtained which were simultaneously unable to differentiate into myotubes and exhibited altered morphology. These characteristics were retained by the variants when subcultured in the absence of the drug for over 700 generations. Several of the azacytidine resistant cells were more susceptible than the parental line to the lethal action of 5-bromodeoxyuridine and adenosine, but not that of cytosine arabinoside, ouabain or 8-azaguanine. The variants were capable of transporting uridine, thymidine and 5-azacytidine. The uridine kinase activity was one-half to one-third of than in the parental cells but it was not missing completely in any of the variants. Two independently isolated variants selected for detailed study showed a 2- to 3-fold increase in the activity of orotidylic acid decarboxylase. This enzyme in the variants in contrast to that of the parental cells was completely insensitive to the inhibitory effect of a nucleotide generated from ATP and 5-azacytidine in cell extracts (probably 5-azacytidine m...Continue Reading

References

May 3, 1976·Biochemical and Biophysical Research Communications·R WhatleyB D Sanwal
Apr 25, 1975·Journal of Molecular Biology·C P Emerson, S K Beckner
Feb 23, 1973·Biochimica Et Biophysica Acta·M ReichmanS Penman
Oct 1, 1968·Journal of Cellular Physiology·J R Coleman, A W Coleman
Oct 22, 1969·Biochimica Et Biophysica Acta·T L SteckJ P Bader
Jan 1, 1969·Current Topics in Developmental Biology·D Yaffe
Feb 27, 1974·Biochemical and Biophysical Research Communications·S GülenG C Tremblay
May 1, 1971·Developmental Biology·A ShainbergD Yaffe
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W F LoomisD Luzzati
Nov 1, 1972·Experimental Cell Research·G E Morris, R J Cole
May 15, 1973·Biochemical and Biophysical Research Communications·J P WahrmannR Winand
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D Yaffe
Nov 1, 1965·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K Okazaki, H Holtzer
Feb 1, 1969·Journal of Cellular Physiology·M O'Neill, R C Strohman
Jul 1, 1958·The Anatomical Record·H HOLTZER, J ABBOTT
Jun 1, 1960·The Journal of Biophysical and Biochemical Cytology·C R CAPERS
Sep 1, 1961·Experimental Cell Research·F E STOCKDALE, H HOLTZER
Jun 21, 1963·Science·I R KONIGSBERG

❮ Previous
Next ❯

Citations

Sep 1, 1978·Developmental Biology·P G ConstantinidesP A Jones
Jan 1, 1979·The International Journal of Biochemistry·W J TaxJ H Veerkamp
Oct 16, 1980·Biochemical and Biophysical Research Communications·B M Gilfix, B D Sanwal
Jan 1, 1980·Journal of Cellular Physiology·E H Ball, B D Sanwal
Dec 1, 1983·Molecular and Cellular Biology·B GilfixB D Sanwal
Jun 30, 1988·Biochimica Et Biophysica Acta·A E JacobsJ H Veerkamp
Jan 1, 1984·Differentiation; Research in Biological Diversity·C Walker, J W Shay

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.