Alterations in gene expression levels provide early indicators of chemical stress during Xenopus laevis embryo development: A case study with perfluorooctane sulfonate (PFOS)

Ecotoxicology and Environmental Safety
Laura San-SegundoM Victoria Pablos

Abstract

In the present study, Xenopus laevis embryos were exposed to a range of perfluorooctane sulfonate (PFOS) concentrations (0, 0.5, 6, 12, 24, 48 and 96mg/L) for 96h in laboratorial conditions to establish toxicity along with possible gene expression changes. Mortality and deformities were monitored daily and head-tail length was measured at the end of the assay as an indicator of growth. At 24 and 96h post-exposure (hpe), the mRNA expression levels of the genetic markers involved in general stress responses (hsp70, hsp47, crh-a and ucn1), oxidative stress (cat.2 and sod), lipid metabolism (ppard) and apoptosis (tp53 and bax) were analyzed by RT-qPCR. Malformations were significantly higher in the embryos exposed to the highest PFOS concentration (41.8% to 56.4%) compared to controls (5.5%) at 48, 72 and 96hpe. Growth inhibition was observed in the embryos exposed to PFOS concentrations≥48mg/L. At 24 hpe, a statistically significant up-regulation of genes hsp70, hsp47, ppard, tp53 and bax in relation to controls was found. Similar responses were found for genes hsp70, hsp47, crh-a, ucn1, sod and ppard at 96 hpe. Alterations in the mRNA expression levels indicated both a stress response to PFOS exposure during X. laevis embryo deve...Continue Reading

References

Feb 21, 1998·Ecotoxicology and Environmental Safety·E PyzaR Laskowski
Apr 3, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·K Nagata
Feb 16, 2005·Annual Review of Physiology·Dietmar Kültz
Jan 3, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Amanda M Hamilton, John J Heikkila
Jan 18, 2006·General and Comparative Endocrinology·Graham C Boorse, Robert J Denver
Apr 18, 2006·Aquatic Toxicology·F MouchetL Gauthier
Jul 22, 2006·Environmental Science & Technology·Gerald T AnkleyDonald Versteeg
Mar 31, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jaishri Menon, Roy Rozman
Apr 15, 2008·Toxicology and Applied Pharmacology·Xiongjie ShiBingsheng Zhou
Apr 22, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Anne Vatland KrøvelPål A Olsvik
Jun 5, 2008·Antioxidants & Redox Signaling·Dunyaporn TrachoothamPeng Huang
Jul 3, 2008·Environmental Science and Pollution Research International·Monica SáezJohn R Parsons
Aug 21, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Cynthia J WolfBarbara D Abbott
Apr 28, 2009·General and Comparative Endocrinology·Robert J Denver
Jul 2, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Steven O SimmonsRam Ramabhadran
Jan 12, 2010·Life Sciences·Subash C GuptaDebapratim K Chowdhuri
Feb 26, 2010·Cold Spring Harbor Perspectives in Biology·Mary Ellen Perry
May 6, 2011·Environmental Science & Technology·Magali HoudeRobert J Letcher
Dec 23, 2011·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Laura GuimarãesLúcia Guilhermino
May 3, 2013·Environmental Toxicology and Chemistry·Laura San SegundoM Victoria Pablos
Feb 13, 2014·Molecular Therapy. Nucleic Acids·Véronique BolducCarsten G Bönnemann
Apr 25, 2014·Cellular and Molecular Life Sciences : CMLS·Rayees U H Mattoo, Pierre Goloubinoff
Jan 23, 2015·Environmental Science and Pollution Research International·María Victoria PablosCarlos Fernández

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Feb 24, 2021·Environmental Pollution·Miguel González-DoncelEulalia María Beltrán

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