PMID: 6171670Oct 1, 1981Paper

Alterations in glomerular RNA in diabetic rats: roles of glucagon and insulin

Kidney International
P CortesN W Levin

Abstract

Incorporation in vivo of labeled orotate into RNA and total nucleotides was measured in isolated glomeruli and whole renal cortex. In 2-day diabetic animals, glomerular RNA was increased, and there was greater incorporation of orotate into total nucleotides and RNA as compared with controls. Insulin reversed the exaggerated incorporation at infusion rates that corrected hyperglucagonemia without reducing plasma glucose and with only minimal changes in insulin concentrations. The addition of glucagon to insulin infusions reproduced the increased incorporation observed in untreated diabetics. Similar changes occurred in renal cortex, where differences in orotate incorporation into nucleotide precursors seemed to be the main cause for alterations in RNA labeling. Isotope incorporation in glomeruli correlated positively with plasma glucagon, but not with insulin or glucose concentrations. Although in 7-month diabetic animals orotate incorporation into RNA was less than in controls, probably as a consequence of renal disease, 24-hour insulin infusion decreased it further. Our results confirm that in the diabetic kidney, abnormal uracil nucleotide metabolism and increased cellular content of RNA are demonstrable in glomeruli as in th...Continue Reading

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Citations

Nov 1, 1986·Clinics in Endocrinology and Metabolism·G C Viberti, M J Wiseman
Nov 1, 1992·The Journal of Clinical Investigation·B L RiserR G Narins
Apr 1, 1983·Kidney International·K Seyer-Hansen
Apr 1, 1988·Kidney International·A M KumarA Spitzer
Aug 20, 2010·Journal of Pharmaceutical and Biomedical Analysis·Claudia BalderasCoral Barbas
Mar 1, 1988·Diabetes/metabolism Reviews·K S Kleinman, L G Fine
Oct 1, 1990·Diabetes Research and Clinical Practice·J L LoganS M Lee
Apr 20, 2006·Molecular and Cellular Biochemistry·Mohammad Rizwan SiddiquiNajma Zaheer Baquer
Dec 1, 1988·The American Journal of Physiology·F Dumler, P Cortes

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