Alterations in hippocampal expression of SNAP-25, GAP-43, stannin and glial fibrillary acidic protein following mechanical and trimethyltin-induced injury in the rat

Neuroscience
C M PatanowM L Billingsley

Abstract

A set of well-defined antisera against neuronal and glial proteins were used to characterize patterns of protein expression in rat hippocampus following transection of the fimbira-fornix and perforant pathways or after administration of the selective neurotoxicant trimethyltin (8 mg/kg, i.p.). SNAP-25 (synaptosomal protein, mol. wt 25,000) is a neuron-specific, developmentally regulated presynaptic protein, stannin is a protein enriched in cells sensitive to trimethyltin, and GAP-43 (growth-associated protein, mol. wt 43,000) is associated with axonal growth and regeneration. Glial fibrillary acidic protein is an astrocyte-specific intermediate filament protein and a marker for reactive gliosis. SNAP-25 immunoreactivity was altered following both neurotoxicant and mechanical injury. Three days after fimbria-fornix/perforant path lesions, there was a loss of SNAP-25 immunoreactivity in hippocampal efferent pathways and in the lesioned entorhinal cortex. By day 12, there was evidence of reinnervation of hippocampal subfields by SNAP-25-immunopositive commissural afferent fibers. On day 3, immunoblots showed the appearance of SNAP-25a, a developmental isoform produced by alternative splicing of nine amino acids in exon 5, in lesio...Continue Reading

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Citations

Jun 1, 2001·Toxicon : Official Journal of the International Society on Toxinology·C V JurasinskiC L Schengrund
Jun 6, 2000·Journal of Neuroscience Methods·A C ScalletJ C Matthews
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Aug 4, 2009·Hippocampus·Jerome L Rekart, Aryeh Routtenberg
Aug 30, 2014·Behavioural Pharmacology·Francisco Diego Pinheiro FernandesGeanne Matos de Andrade
Jun 30, 2019·Molecular Psychiatry·Vijayasree V GiridharanTatiana Barichello
Nov 6, 2004·The Journal of Biological Chemistry·Malin K Sandberg, Peter Löw
May 26, 2009·Neurotoxicology·Ana-Maria Florea, Dietrich Büsselberg

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