Alterations in ileal mucosa bacteria related to diet complexity and growth performance in young pigs

PloS One
Crystal L LevesqueKees de Lange

Abstract

Evaluation of the prolonged impact of weaning diet on ileal mucosa bacteria and during periods of reduced and improved growth was conducted using 454 pyrosequencing. Weaned pigs were fed HIGH or LOW complexity diets, with or without antibiotics, for 6 weeks, followed by a common grower diet. Pigs were killed at 2 (n = 4 or 5) and 8 (n = 6) weeks post-weaning (periods of reduced and improved growth, respectively). Mucosal bacteria were removed; DNA was extracted and amplified using the V1-V3 region of the 16S rRNA gene. Mucosal bacteria clustered more closely by week post-weaning than diet but 44% of bacterial species did not change from week 2 to 8. There was no effect of diet complexity or antibiotic inclusion on indices of bacterial diversity. Firmicutes made up 91 and 96% of total reads at week 2 and 8, respectively. The proportion of Clostridium paraputrificum increased (P = 0.003) from week 2 to 8 in pigs fed LOW but didn't change in pigs fed HIGH; whereas Clostridium leptum decreased (P = 0.02) from week 2 to 8 in pigs fed LOW but didn't change in pigs fed HIGH. The proportion of Sarcina genus was 3-fold higher in pigs fed A+ compared to A- at week 2 and 5-fold higher at week 8 despite the lack of in-feed antibiotics at t...Continue Reading

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Citations

Jun 2, 2017·MSystems·Devin B HolmanHeather K Allen
Apr 27, 2019·PloS One·Emma T HelmNicholas K Gabler
Feb 7, 2020·Animal : an International Journal of Animal Bioscience·J OrtmanC L Levesque
May 18, 2018·Translational Animal Science·Crystal L LevesqueJulang Li
Jul 19, 2020·Animals : an Open Access Journal From MDPI·Laurynas VadopalasElena Bartkiene
Mar 2, 2021·Frontiers in Veterinary Science·Lan ZhengSung Woo Kim

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Methods Mentioned

BETA
electrophoresis
PCR
chip
454 Genome Sequencing
PCA

Software Mentioned

SAS
BLASTn

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