PMID: 8974070Oct 1, 1996Paper

Alterations in inotropy, nitric oxide and cyclic GMP synthesis, protein phosphorylation and ADP-ribosylation in the endotoxin-treated rat myocardium and cardiomyocytes

Molecular and Cellular Biochemistry
P V SulakheR R Mainra

Abstract

To evaluate the effects of the in vivo endotoxin treatment of the rat on (1) the contractile responses in the subsequently isolated papillary muscle to adrenergic and cholinergic agonists and (2) the biochemical parameters (cyclic GMP, nitric oxide synthesis, protein phosphorylation and ADP-ribosyslation) in the subsequently isolated cardiomyocytes. Following the in vivo endotoxin treatment (4 mg/kg i.p., 18 h), contractile responses to increasing amounts of isoprenaline or to increasing amounts of oxotremorine in the presence of a fixed amount of isoprenaline were determined in isolated papillary strips. Activities of nitric oxide synthase, guanylyl cyclase, as well as phosphorylation of phospholamban and troponin-inhibitory subunit, and pertussis toxin-catalyzed and endogenous ADP-ribosylations were determined in the intact cardiomyocytes and subcellular fractions. The increase in the force of contraction by isoprenaline was reduced, while its inhibition by oxotremorine was greater in the endotoxin-treated papillary strips. The activities of both nitric oxide synthase, primarily of the inducible form of the enzyme, and cytosolic guanylyl cyclase were higher while the phosphorylations of both phospholamban and troponin-inhibit...Continue Reading

References

Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P F MéryR Fischmeister
Jul 31, 1990·European Journal of Pharmacology·A P BraunP V Sulakhe
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T GulickG F Schreiner
Aug 3, 1989·The New England Journal of Medicine·A F SuffrediniJ E Parrillo
Jun 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·W J GeorgeN D Goldberg
Jan 1, 1982·Annual Review of Physiology·M Tada, A M Katz
Mar 1, 1994·Pflügers Archiv : European journal of physiology·R C LeviM P Gallo
Jan 1, 1995·The American Journal of Physiology·G M Wahler, S J Dollinger
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J L BalligandT Michel
Aug 18, 1993·Biochimica Et Biophysica Acta·H H SchmidtU Walter
Feb 1, 1995·The Journal of Clinical Investigation·M KirsteinR Fischmeister
Sep 1, 1994·Molecular and Cellular Biochemistry·L J McDonald, J Moss
Apr 15, 1994·The Journal of Physiology·X HanW R Giles
Feb 1, 1994·Journal of Molecular and Cellular Cardiology·E E QuistM K Jacobson
May 1, 1994·Circulation Research·A M ShahE G Lakatta
Jan 1, 1994·Life Sciences·B BrüneE G Lapetina
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L J McDonald, J Moss

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Citations

Oct 6, 1999·American Journal of Respiratory and Critical Care Medicine·N Abi-GergesP F Méry
Nov 10, 2015·The Journal of Critical Care Medicine·Andrei SchwartzMoti Klein
Aug 10, 2019·Herzschrittmachertherapie & Elektrophysiologie·Benjamin RathLars Eckardt
Aug 16, 2001·Journal of Cardiothoracic and Vascular Anesthesia·A KumarJ E Parrillo
Feb 15, 2003·European Journal of Pharmacology·Rebecca H RitchieGregory J Dusting

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