Alterations in intracellular and extracellular activities of antioxidant enzymes during suspension culture of sweetpotato

Phytochemistry
Young-Hwa KimGyung-Hye Huh

Abstract

Cultured plant cells are a good system for the study of antioxidant mechanisms and for the mass production of antioxidants, because they can be grown under conditions of high oxidative stress. Alterations in the intracellular and extracellular activities of three antioxidant enzymes, superoxide dismutase (SOD), guaiacol-type peroxidase (POD), and glutathione peroxidase (GPX), were investigated in suspension cultures of sweetpotato (Ipomoea batatas) during cell growth. Intracellular SOD activities (units/mg protein) at 15 days after subculture (DAS) and 30 DAS were 10 and 20 times higher, respectively, compared with the SOD activity at 1 DAS, whereas intracellular specific POD and GPX activities did not significantly increase until after 15 DAS, when they rapidly increased. The extracellular activities of the three enzymes in culture medium were much higher than were the intracellular activities. The change in extracellular SOD activity was similar to that of extracellular GPX during cell growth. Those activities showed high levels until 5 DAS and then significantly decreased. Extracellular POD activity had an almost constant level regardless of the cell growth stage. In addition, intracellular SOD and POD isozymes were quite di...Continue Reading

References

Jan 1, 1978·Methods in Enzymology·A L Tappel
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D BuffardR Esnault
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Mar 31, 1999·The Plant Journal : for Cell and Molecular Biology·H LeeJ K Zhu
Feb 8, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·T AdachiX L Wang
Jul 13, 2000·Cellular and Molecular Life Sciences : CMLS·J DatF Van Breusegem
Jan 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S A ComhairS C Erzurum
Sep 18, 2002·Trends in Plant Science·Ron Mittler
Apr 3, 2003·Biochemical and Biophysical Research Communications·Tomomi OokawaraKeiichiro Suzuki
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Kozi Asada

❮ Previous
Next ❯

Citations

Dec 4, 2008·Applied Biochemistry and Biotechnology·Milen GeorgievMaria Angelova
Feb 22, 2012·Applied Biochemistry and Biotechnology·Nuria González-RábadeJesús Agustín Badillo-Corona
Apr 7, 2009·Journal of Plant Physiology·Penélope García-AnguloJesús M Álvarez
Jun 20, 2016·Plant Physiology and Biochemistry : PPB·Asier Largo-GosensJesús M Álvarez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.