Alterations in intracellular calcium activity and contractility of isolated perfused rabbit hearts by ionophores and adrenergic agents

Biochemical and Biophysical Research Communications
F A Lattanzio, B C Pressman

Abstract

The fluorescent calcium indicator quin2 has been used for the first continuous measurement of the effects of pharmacological agents on intracellular calcium activity in isolated, perfused rabbit hearts. The average intracellular calcium activity was elevated after the infusion of norepinephrine, concurrent with increases in left ventricular pressure and heart rate. These changes were abolished by pretreatment of the heart with phentolamine and nadolol, alpha and beta adrenergic receptor antagonists, respectively. Pretreatment with phentolamine and nadolol did not eliminate the increases in left ventricular pressure and intracellular calcium activity caused by the infusion of the monovalent carboxylic ionophores monensin and salinomycin. It is concluded that the ionophores cause these effects by elevating intracellular sodium activity, which then raises the intracellular calcium activity of the myocardium through intracellular displacement and/or transcellular exchange. It is suggested that the use of fluorescent calcium indicators in intact organs could be useful in evaluating the role of calcium in a variety of pathological states.

References

Jan 1, 1979·Journal of Cardiovascular Pharmacology·R K SainiB C Pressman
Dec 30, 1975·Annals of the New York Academy of Sciences·B C Pressman, N T deGuzman
Apr 1, 1985·Cell Calcium·T J Rink, T Pozzan
Jan 1, 1982·Progress in Biophysics and Molecular Biology·J R BlinksF G Prendergast

❮ Previous
Next ❯

Citations

Aug 25, 1995·European Journal of Pharmacology·Y HottaJ Sakakibara
May 7, 1990·Biochimica Et Biophysica Acta·H H MollenhauerL D Rowe
Feb 1, 1994·Journal of Pharmacological and Toxicological Methods·H Hayashi, H Miyata
Jun 3, 2011·Cell Death & Disease·W Boehmerle, M Endres
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Desheng LuDennis A Carson
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E MarbanV P Chacko
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H C LeeW T Clusin
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H L KirschenlohrG A Smith
Oct 31, 2001·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·C CaldeiraE A Burdmann
Apr 1, 1988·American Heart Journal·J Wikman-CoffeltD T Mason
Jan 1, 1989·British Journal of Clinical Pharmacology·W W Parmley
Sep 1, 1988·Circulation Research·E MarbanM M Pike
Mar 5, 1998·The American Journal of Physiology·P J Del NidoA P Koretsky
Aug 3, 2017·International Journal of Oncology·Justin W Magrath, Yonghyun Kim
Sep 1, 1990·The Journal of Surgical Research·E JimenezS Levitsky

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.