Alterations in levels of DnaK and GroEL result in diminished survival and adherence of stressed Haemophilus ducreyi.

Infection and Immunity
L M ParsonsM Shayegani

Abstract

Haemophilus ducreyi is a hemin-requiring bacterium causing the genital ulcer disease chancroid. Previously we demonstrated that the heat shock protein GroEL was immunogenic and possibly highly expressed in a mammalian host. The present study was initiated to (i) determine the relative amounts of GroEL expressed by H. ducreyi during in vitro exposure to stresses and (ii) evaluate whether a high level of GroEL is directly or indirectly required for survival and adherence of stressed H. ducreyi. Using scanning densitometry of sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profiles, we found that H. ducreyi expressed high basal levels of GroEL, averaging fivefold greater than in Escherichia coli. These high GroEL levels increased up to twofold upon exposure of the organism to heat shock or high levels of hydrogen peroxide and during adherence to two human genital cell lines. Furthermore, when the gene for DnaK was present on a multicopy plasmid in H. ducreyi, a 1.8-fold increase in DnaK and a 2.3-fold reduction in GroEL were seen. These results suggest that DnaK serves as a negative modulator of H. ducreyi GroEL. Subsequently we found that H. ducreyi with lower GroEL had diminished ability to survive when challen...Continue Reading

References

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Citations

Dec 24, 2002·Infection and Immunity·Barbara AlbigerAnn-Beth Jonsson
Dec 24, 2008·Journal of Bacteriology·Jeremiah J Davie, Anthony A Campagnari
Oct 16, 2002·Infection and Immunity·Leah E ColeChristopher Elkins
Feb 13, 2001·Microbiology·C HennequinT Karjalainen
Apr 26, 2017·Journal of Industrial Microbiology & Biotechnology·Yukai SuoJufang Wang
Nov 13, 1998·Journal of Bacteriology·C K WardE J Hansen
Jan 23, 1999·Infection and Immunity·B A BauerE J Hansen
Jan 18, 2002·Infection and Immunity·Hinda J AhmedTeresa Lagergård
Jun 29, 2002·Journal of Clinical Microbiology·Satheesh NairMartin Altwegg
Nov 4, 2006·Journal of Proteome Research·Bing ZhangNagiza F Samatova

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