Alterations in mitochondrial function and spermatozoal motility in goat spermatozoa following incubation with a human lysozyme plasmid

Animal Reproduction Science
Huiming MaYong Zhang

Abstract

Spermatozoa mediated gene transfer has become a promising technology to generate transgenic animals with disease resistance. However, exogenous DNA invasion may cause changes in spermatozoon natural defense system which result in spermatozoon dysfunction.The objective of this study was to investigate the changes of mitochondrial function and motility in goat spermatozoa after pre-incubation and incubation with and without the human lysozyme plasmid pFLAG-hLY. The results demonstrated that human lysozyme plasmid pFLAG-hLY could bind to the surface of the spermatozoon membrane at 186,000 copies/spermatozoa and incorporate to spermatozoon nucleus at 78 copies/spermatozoa after incubation. However, the treated spermatozoon samples showed a significant lower motility (29.7+/-2.2% vs. pre-incubation control 48.0+/-1.4% and incubation control 54.5+/-1.5%, P < 0.05), and percentage of rapid progressive motile spermatozoa(16.4+/-2.4% vs. pre-incubation control 31.4+/-0.6% and incubation control 37.0+/-0.5%,P < 0.01). Meanwhile, the incubation with plasmids caused significant reduction of mitochondrial membrane potential (31.44+/-2.17% vs. pre-incubation control 51.79+/-2.08% and incubation control 58.81+/-1.76%, P < 0.05). In addition, ...Continue Reading

References

Mar 1, 1992·Molecular Reproduction and Development·M LavitranoC Spadafora
Jan 1, 1996·Reproduction, Fertility, and Development·F GandolfiM Courot
Dec 17, 2002·Current Opinion in Biotechnology·Louis Marie Houdebine
Jan 28, 2003·Molecular Reproduction and Development·Marialuisa LavitranoEraldo Seren
Sep 3, 2003·Pharmacology & Therapeutics·Jianglin Fan, Teruo Watanabe
Apr 5, 2005·Nature Biotechnology·Robert J WallHarold W Hawk
Aug 2, 2005·Theriogenology·M Anzar, M M Buhr
Oct 26, 2005·Transgenic Research·David M DonovanRobert J Wall
Mar 3, 2006·Neurochemistry International·Tanusree SenSasanka Chakrabarti
Oct 13, 2006·Annual Review of Pharmacology and Toxicology·Sten OrreniusBoris Zhivotovsky
Jun 3, 2008·Toxicology and Applied Pharmacology·Niraj PantDaya Krishna Saxena
Apr 18, 2009·Toxicology and Applied Pharmacology·Cecilia Eliana Hanzel, Sandra Viviana Verstraeten

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Citations

Apr 25, 2012·Transgenic Research·Laurent BoulangerJean-Luc Vilotte
Feb 24, 2015·Theriogenology·Mojtaba ShafieiMohammad Hossein Nasr Esfahani
Jun 3, 2020·Zygote : the Biology of Gametes and Early Embryos·Linlin SunWenxian Zeng
Mar 7, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jian GaoJianxin Chen

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