Alterations in opsonophagocytic killing by neutrophils of Bacteroides fragilis associated with animal and laboratory passage: effect of capsular polysaccharide

The Journal of Infectious Diseases
G L SimonS L Gorbach

Abstract

Electron microscopic studies of laboratory-passaged and animal-passaged isolates of Bacteroides fragilis showed significantly more capsular material around the latter. This observation correlated with increased survival of animal-passaged bacteria in rabbit intraperitoneal chambers and increased resistance to phagocytosis and opsonophagocytic killing by neutrophils. With an initial inoculum of 2.5 x 10(6) colony-forming units/ml, the number of bacteria surviving after incubation for 2 hr with neutrophils and pooled normal human serum was significantly (P less than 0.01) greater for animal-passaged than for laboratory-passaged bacteria. Neutrophil uptake of 14C-labeled animal-passaged and laboratory-passaged bacteria after incubation for 20 min was 45% and 63%, respectively (P less than 0.05). No significant difference in survival was found between animal-passaged and laboratory-passaged isolates of Bacteroides thetaiotaomicron. These findings suggest that on mechanism whereby the capsule of B. fragilis contributes to virulence is by inhibiting opsonophagocytic killing.

Citations

Jan 1, 1987·Archives of Gynecology and Obstetrics·I Brook
Jan 1, 1984·Antonie van Leeuwenhoek·D M MacLarenJ A Kaan
Feb 1, 1986·Diagnostic Microbiology and Infectious Disease·M S Topiel, G L Simon
Oct 16, 2007·Clinical Microbiology Reviews·Hannah M Wexler
Jan 1, 1994·FEMS Microbiology Reviews·I Brook
Jan 1, 1986·Antonie van Leeuwenhoek·I BrookR I Walker
Dec 1, 1990·The British Journal of Oral & Maxillofacial Surgery·M A LewisD A McGowan
Jan 1, 1984·Scandinavian Journal of Infectious Diseases. Supplementum
May 1, 1995·The Journal of Infection·I Brook, J D Gillmore
Mar 13, 2018·Annals of the New York Academy of Sciences·Deniz Erturk-Hasdemir, Dennis L Kasper
Jan 1, 1987·Critical Reviews in Microbiology·I Brook
Dec 21, 2019·Journal of Molecular Biology·Ezequiel Valguarnera, Juliane Bubeck Wardenburg

Related Concepts

Metazoa
Bacteroides fragilis
Bacteroides Infections
Blood Bactericidal Activity
Isoferritin
Neutrophil Band Cells
Opsonin
Phagocytosis
Polysaccharides, Bacterial
Chinchilla Rabbits

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.