PMID: 375990Mar 22, 1979Paper

Alterations in pancreatic islet phosphate content during secretory stimulation with glucose

Biochimica Et Biophysica Acta
L BukowieckiN Freinkel

Abstract

Isolated rat pancreatic islets were perifused and analyzed for phosphate content immediately following the transient increase in the efflux of orthophosphate which occurs when insulin secretion is stimulated by glucose. In some instances, islets were perifused directly following isolation to minimize preparative delay; in others, islets were prelabeled during incubation with [32P]orthophosphate for 90 min prior to perifusion. In both experimental situations, total islet phosphate content declined 40--50% following exposure to stimulating concentrations of glucose and initiation of enhanced insulin release. In the experiments with prelabeled islets, tissue content of [32P]orthophosphate fell to a similar extent so that the specific radioactivity of islet orthophosphate was unaffected. Inhibited of heightened insulin release with Ni2+ did not modify the decrements in total or radioactive tissue orthophosphate, thus indicating that these responses to islet stimulation reflect events which are proximal to activated exocytosis. Simultaneous analyses for tissue ATP and ADP demonstrated that the efflux in orthophosphate and reduction in tissue orthophosphate content were not mediated via net changes in islet adenine nucleotides. The o...Continue Reading

References

Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·N FreinkelR M Dawson
Mar 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A U Parman
Feb 1, 1973·The Biochemical Journal·S J AshcroftP J Randle
Jan 1, 1972·Diabetes·F M MatschinskyJ Kotler-Brajtburg
Oct 1, 1973·Diabetologia·L A Idahl
Nov 1, 1974·The Journal of Clinical Investigation·N FreinkelR M Dawson
Feb 1, 1938·The Biochemical Journal·I Berenblum, E Chain

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Citations

Aug 14, 2013·Molecular and Cellular Endocrinology·Xianglan QuanKyu-Sang Park
Jun 28, 1985·Biochemical and Biophysical Research Communications·R C Johnson, N Freinkel
Apr 13, 2018·Journal of Applied Physiology·David F WilsonFranz M Matschinsky
Jan 6, 1983·Nature·J C HenquinM Cogneau
Aug 28, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tuyet Thi NguyenKyu-Sang Park
Jul 1, 1980·Acta Pathologica Et Microbiologica Scandinavica. Section A, Pathology·L Boquist
Apr 17, 2019·Journal of Applied Physiology·David F Wilson, Franz M Matschinsky
Jan 1, 1989·The American Journal of Physiology·M D MeglassonM Erecinska
Aug 23, 2020·Diabetes·Christopher J BarkerPer-Olof Berggren
May 1, 1986·The American Journal of Physiology·M HoenigF M Matschinsky
Aug 7, 1992·Biochimica Et Biophysica Acta·M ErecińskaD Nelson
Dec 1, 1989·Metabolism: Clinical and Experimental·M D MeglassonM Erecinska

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