PMID: 5902571Jan 1, 1966

Alterations in protein and nucleic acid metabolism of lymphoma 6C3HED-og cells in mice given guinea pig serum

The Journal of Experimental Medicine
L H SOBIN, J G KIDD

Abstract

Lymphoma 6C3HED-OG cells, known from previous work to be susceptible to the effects of guinea pig serum in vivo and dependent upon extrinsic asparagine for protein synthesis and growth in vitro, remained for the most part morphologically intact and countable in the electronic cell counter following exposures of 1 and 2 hr to the effects of heated (56 degrees C, 30 min) guinea pig serum injected into the peritoneal cavities of mice in which the lymphoma cells were growing rapidly; after exposures of 4 and 6 hr the bulk of the -OG cells remained still intact and countable in the cell counter, though by this time a small proportion of them (5 to 12%) proved stainable with eosin in wet preparations) hence were presumably nonviable. After 12, 16, and 24 hr of exposure, however, the bulk of the -OG cells were either lysed or fragmented, to the extent that they did not register in the cell counter. Morphologic studies of the cells exposed 16 and 24 hr to the effects of heated guinea pig serum in vivo, disclosed that most of the cells then remaining were either frankly necrotic or greatly altered otherwise, marked vacuolation of the cytoplasm being the most conspicuous alteration in cells not yet obviously necrotic. Long before the bul...Continue Reading

References

Jun 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·N D HOLMQUIST
Nov 16, 1963·Nature·M R SIEGEL, H D SISLER
Jan 1, 1964·Proceedings of the Society for Experimental Biology and Medicine·S PERRY, J C MARSH
May 1, 1964·The Journal of Cell Biology·G E STONE, D M PRESCOTT
May 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·M K PATTERSONT A MCCOY
Jun 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·L H SOBIN, J G KIDD

Citations

Nov 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·J H SchwartzJ D BROOME
Jan 15, 1969·Klinische Wochenschrift·E Grundmann
Jan 15, 1971·Nature·D Crowther
Apr 5, 1968·Biochemical and Biophysical Research Communications·M D Prager, N Bachynsky
Sep 20, 1968·Archives of Biochemistry and Biophysics·M D Prager, N Bachynsky
Sep 24, 1970·FEBS Letters·D Kessel, H B. Bosmann
Jul 1, 1967·Cancer·L J OLDJ D Teller

Related Concepts

Blood
Carbon Isotopes
DNA, Neoplasm
Cavia porcellus
Lymphoma
Neoplasm Proteins
RNA, Neoplasm
Thymidine
Transplantation Immunology
Tritium

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