Hemodynamic conditions play a critical role in embryonic cardiovascular development, and altered blood flow leads to congenital heart defects. Chicken embryos are frequently used as models of cardiac development, with abnormal blood flow achieved through surgical interventions such as outflow tract (OFT) banding, in which a suture is tightened around the heart OFT to restrict blood flow. Banding in embryos increases blood pressure and alters blood flow dynamics, leading to cardiac malformations similar to those seen in human congenital heart disease. In studying these hemodynamic changes, synchronization of data to the cardiac cycle is challenging, and alterations in the timing of cardiovascular events after interventions are frequently lost. To overcome this difficulty, we used ECG signals from chicken embryos (Hamburger-Hamilton stage 18, ∼3 days of incubation) to synchronize blood pressure measurements and optical coherence tomography images. Our results revealed that, after 2 h of banding, blood pressure and pulse wave propagation strongly depend on band tightness. In particular, while pulse transit time in the heart OFT of control embryos is ∼10% of the cardiac cycle, after banding (35% to 50% band tightness) it becomes ne...Continue Reading
Effect of conotruncal constriction on aortic-mitral valve continuity in the stage 18, 21 and 24 chick embryo
Unilateral vitelline vein ligation alters intracardiac blood flow patterns and morphogenesis in the chick embryo
Remodeling of chick embryonic ventricular myoarchitecture under experimentally changed loading conditions
Rate of coronary vascularization during embryonic chicken development is influenced by the rate of myocardial growth
Distinct regulation of vascular endothelial growth factor in intact human conduit vessels exposed to laminar fluid shear stress and pressure
Regional passive ventricular stress-strain relations during development of altered loads in chick embryo
Simultaneous electrocardiograms and myograms of the isolated atrium, ventricle and conus of the embryonic chick heart
Changes in shear stress-related gene expression after experimentally altered venous return in the chicken embryo
Changes in wall motion and blood flow in the outflow tract of chick embryonic hearts observed with optical coherence tomography after outflow tract banding and vitelline-vein ligation
Measurement of absolute blood flow velocity in outflow tract of HH18 chicken embryo based on 4D reconstruction using spectral domain optical coherence tomography
Biomechanics of the chick embryonic heart outflow tract at HH18 using 4D optical coherence tomography imaging and computational modeling
Blood flow dynamics reflect degree of outflow tract banding in Hamburger-Hamilton stage 18 chicken embryos
Blood flow through the embryonic heart outflow tract during cardiac looping in HH13-HH18 chicken embryos
Pulse wave velocity in the microcirculation reflects both vascular compliance and resistance: Insights from computational approaches
Changes in dynamic embryonic heart wall motion in response to outflow tract banding measured using video densitometry
Characterisation of the developing heart in a pressure overloaded model utilising RNA sequencing to direct functional analysis.
Validating the Paradigm That Biomechanical Forces Regulate Embryonic Cardiovascular Morphogenesis and Are Fundamental in the Etiology of Congenital Heart Disease
Organ Dynamics and Fluid Dynamics of the HH25 Chick Embryonic Cardiac Ventricle as Revealed by a Novel 4D High-Frequency Ultrasound Imaging Technique and Computational Flow Simulations
Increased Hemodynamic Load in Early Embryonic Stages Alters Myofibril and Mitochondrial Organization in the Myocardium
Effect of left atrial ligation-driven altered inflow hemodynamics on embryonic heart development: clues for prenatal progression of hypoplastic left heart syndrome.
Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.